Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax
Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax
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DOI：
10.1016/s0001-706x(97)00677-3
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发表时间：
1997-06-24
期刊：
影响因子：
2.7
通讯作者：
Geerts, S
中科院分区：
文献类型：
--
作者：
deAlmeida, PJLP;Ndao, M;Geerts, S
用间日锥虫原种对6只山羊进行了实验性感染。在给药后15周和另外3周期间，每周通过血沉棕黄层和湿血膜检查监测寄生虫血症。使用Chelex（R）100（BioRad）的提取方法，通过聚合酶链反应（PCR）测试干燥的血液样品。PCR证明一贯比寄生虫学技术更敏感。(C)1997年Elsevier Science B.V.
Six goats were experimentally infected with a stock of Trypanosoma vivax. Parasitaemia was weekly monitored by buffy coat and wet blood film examination during a period of 15 weeks and another 3 weeks following drug-treatment. Dried blood samples were tested by the polymerase chain reaction (PCR), using an extraction method with Chelex(R) 100 (BioRad). PCR proved consistently more sensitive than the parasitological techniques. (C) 1997 Elsevier Science B.V.