Superior mineralization and neovascularization capacity of adult human metaphyseal periosteum-derived cells for skeletal tissue engineering applications
Superior mineralization and neovascularization capacity of adult human metaphyseal periosteum-derived cells for skeletal tissue engineering applications
复制标题
成人干骺端骨膜衍生细胞的卓越矿化和新生血管能力，用于骨骼组织工程应用
DOI：
10.3892/ijmm.2011.634
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发表时间：
2011-05-01
影响因子：
5.4
通讯作者：
Huang, Chenglong
中科院分区：
文献类型：
--
作者：
Chen, Daoyun;Shen, Hao;Huang, Chenglong
骨组织工程是一种很有前途的基于细胞的治疗骨缺损的策略。成人骨髓间充质干细胞(HBMSCs)是一种常用的成骨细胞来源。然而，这些干细胞在成人骨髓中的低频率和有限的增殖限制了它们的临床应用。骨髓间充质干细胞的另一种来源是骨膜来源的细胞，这些细胞似乎很容易获得和体外扩增。我们从相同的供体中分离出人干骺端骨膜来源的细胞(HMPC)和hBMSCs，并比较了它们在体外和体内的成骨能力。经单层培养诱导成骨后，hMPC较hBMSCs具有更强的矿化能力，BMP-2、骨桥蛋白和骨钙素的表达水平高于hBMSCs。在免疫缺陷小鼠体内植入细胞-β-磷酸三钙支架8周后，植入hMPC的新生血管和成熟骨形成率均高于hBMSC。综上所述，hMPC是一种很有前途的骨组织工程细胞候选材料。
Bone tissue engineering is a promising cell-based strategy to treat bone defects. Mesenchymal stem cells from adult human bone marrow (hBMSCs) are a frequently used cellular source for bone tissue generation. However, the low frequency of these stem cells in adult bone marrow and their limited proliferation restrict their clinical utility. An alternative source of MSCs is the periosteum-derived cells, and these cells appear to be easy to harvest and expand ex vivo. We isolated human metaphyseal periosteum-derived cells (hMPCs) and hBMSCs from the same donors and compared their osteogenic capacity both in vitro and in vivo. After osteogenic induction in monolayer cultures, hMPCs resulted in more robust mineralization and expressed higher mRNA levels of BMP-2, osteopontin and osteocalcin than hBMSCs. Eight weeks after implantation of cellular-beta-TCP scaffolds in immunodeficient mice, hMPC implantation showed higher neovascularization and higher percentage of mature bone formation than hBMSC implantation. In conclusion, hMPCs represent a promising cellular candidate for bone tissue engineering.