Three-Dimensional Architecture and Cell Composition of a Choukroun's Platelet-Rich Fibrin Clot and Membrane
Three-Dimensional Architecture and Cell Composition of a Choukroun's Platelet-Rich Fibrin Clot and Membrane
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DOI：
10.1902/jop.2009.090531
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发表时间：
2010-04-01
影响因子：
4.3
通讯作者：
Charrier, Jean-Baptiste
中科院分区：
文献类型：
--
作者：
Ehrenfest, David M. Dohan;Del Corso, Marco;Charrier, Jean-Baptiste
背景：富血小板纤维蛋白（PRF; Choukroun技术）是第二代外科用血小板浓缩物。这个简单的协议允许生产白细胞和血小板丰富的纤维蛋白凝块和膜从10毫升的血液样本开始。本研究的目的是确定这种自体生物材料的细胞组成和三维组织，并评估不同的收集管（干玻璃或玻璃涂层的塑料管）和压缩程序（强制或软）对最终的PRF膜architecture.Methods的影响：离心后，血液分析进行收集PRF凝块后的残余废物血浆层。PRF凝块和膜进行处理，通过光学显微镜和扫描电子显微镜检查。结果：约97%的血小板和>50%的白细胞集中在PRF凝块，并显示出特定的三维分布，这取决于离心力。血小板和纤维蛋白在红细胞基底之外的膜的第一毫米内形成大的凝结团。纤维蛋白网络非常成熟和致密。此外，使用不同的测试收集管和压缩技术组之间的PRF架构没有显着差异，即使这两个参数可能会影响生长因子含量和生物基质properties.Conclusions：PRF协议集中了大多数血小板和白细胞从血液收获成一个单一的自体纤维蛋白生物材料。只要遵守主要生产原则，该方案可提供可重现的结果。J Periodontol 2010;81：546-555.
Background: Platelet-rich fibrin (PRF; Choukroun's technique) is a second-generation platelet concentrate for surgical use. This easy protocol allows the production of leukocyte- and platelet-rich fibrin clots and membranes starting from 10-ml blood samples. The purposes of this study were to determine the cell composition and three-dimensional organization of this autologous biomaterial and to evaluate the influence of different collection tubes (dry glass or glass-coated plastic tubes) and compression procedures (forcible or soft) on the final PRF-membrane architecture.Methods: After centrifugation, blood analyses were performed on the residual waste plasmatic layers after collecting PRF clots. The PRF clots and membranes were processed for examination by light microscopy and scanning electron microscopy.Results: Approximately 97% of the platelets and >50% of the leukocytes were concentrated in the PRF clot and showed a specific three-dimensional distribution, depending on the centrifugation forces. Platelets and fibrin formed large clusters of coagulation in the first millimeters of the membrane beyond the red blood cell base. The fibrin network was very mature and dense. Moreover, there was no significant difference in the PRF architecture between groups using the different tested collection tubes and compression techniques, even if these two parameters could have influenced the growth factor content and biologic matrix properties.Conclusions: The PRF protocol concentrated most platelets and leukocytes from a blood harvest into a single autologous fibrin biomaterial. This protocol offers reproducible results as long as the main production principles are respected. J Periodontol 2010;81:546-555.