The Au family, a novel short interspersed element (SINE) from Aegilops umbellulata
The Au family, a novel short interspersed element (SINE) from Aegilops umbellulata
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Au 家族，一种来自伞形山羊草的新型短散布元素 (SINE)
DOI：
10.1007/s001220051668
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发表时间：
2001
影响因子：
5.4
通讯作者：
T. Kawahara
中科院分区：
文献类型：
--
作者：
Y. Yasui;S. Nasuda;Y. Matsuoka;T. Kawahara
摘要：在伞形山羊草（Aegilops umbellulata）乙酰辅酶A羧化酶基因的第二个内含子中鉴定出一种新的植物短散布核元件（SINE），该元件因其发现的宿主物种而被命名为“Au”。 Au 元件具有 tRNA 相关区域、直接侧翼重复序列和 3' 端的短 T 延伸，这些是 Au 和之前表征的 SINE 的共同特征。通过 DNA 点杂交在几种单子叶植物和双子叶植物的基因组中检测到 Au 元件，通过数据库搜索也在烟草基因组中发现 Au 元件。 Au 元件在小麦和AE 中以特别高的拷贝数（每个单倍体基因组大约 104 个拷贝）存在。伞形花序。这表明最近小麦和山羊草中金的含量有所增加。原位杂交揭示了小麦染色体上金元素的分散分布。通过PCR从单子叶植物和双子叶植物中扩增Au元件，并推断Au元件之间的系统发育关系。该系统发育分析表明 Au 元素的扩增方式与 SINE 分散的逆转录转座子模型一致。 Au 元素的高拷贝数及其在小麦中的分散分布是这一重要物种分子标记系统的理想特征。
Abstract A novel plant short interspersed nuclear element (SINE) was identified in the second intron of the acetyl CoA carboxylase gene of Aegilops umbellulata which has been designated ”Au”, for the host species in which it was discovered. Au elements have a tRNA-related region, direct flanking repeats, and a short stretch of T at the 3′ end, which are features common to Au and previously characterized SINEs. Au elements are detected in the genomes of several monocots and dicots by DNA dot hybridization and are also found in the tobacco genome by database searching. Au elements are present at an especially high copy number (approximately 104 copies per haploid genome) in wheat and Ae. umbellulata. This suggests a recent amplification of Au in the Triticum and Aegilops species. In situ hybridization revealed a dispersed distribution of Au elements on wheat chromosomes. Au elements were amplified by PCR from monocot and dicot species and the phylogenetic relationships among Au elements were inferred. This phylogenetic analysis suggests amplification of Au elements in a manner consistent with the retrotransposon model for SINE dispersion. The high copy number of Au elements and their dispersed distribution in wheat are desirable characteristics for a molecular marker system in this important species.