Photoacoustic F-Mode imaging for scale specific contrast in biological systems
Photoacoustic F-Mode imaging for scale specific contrast in biological systems
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DOI：
10.1038/s42005-019-0131-y
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发表时间：
2019-03-19
影响因子：
5.5
通讯作者：
Kolios, Michael C.
中科院分区：
文献类型：
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作者：
Moore, Michael J.;Hysi, Eno;Kolios, Michael C.
在光声（PA）成像中，时域重建技术是当前成像的金标准。虽然这些技术提供了高分辨率的光吸收空间图，但它们忽略了宽带PA信号的频域中编码的结构信息。在这项工作中，我们介绍了一种频域技术PA图像形成，称为F模式。通过利用PA信号的频率内容中包含的信息，F-Mode可用于生成具有特定比例对比度的图像。为了证明我们的技术的鲁棒性，我们将F模式应用于使用PA层析成像和PA显微镜系统获得的数据集，利用中心频率范围为40-400 MHz的线性阵列和单元件换能器。在这里，我们表明，该技术可用于：区分血管和微球的不同大小的幻影，提高可视化的细胞器中培养的细胞，并选择性地显示单血管在体内斑马鱼幼虫。
In photoacoustic (PA) imaging, time domain reconstruction techniques are the current gold standard for image formation. While these techniques provide high-resolution spatial maps of optical absorption, they neglect the structural information encoded in the frequency domain of the broadband PA signals. In this work, we introduce a frequency domain technique for PA image formation, termed F-Mode. By leveraging information contained in the frequency content of PA signals, F-Mode can be used to generate images with scale-specific contrast. To demonstrate the robustness of our technique, we apply F-Mode to datasets acquired using both PA tomography and PA microscopy systems, utilizing linear array and single-element transducers with central frequencies ranging from 40-400 MHz. Here we show that the technique can be used to: differentiate between vessels and microspheres of different size in phantoms, enhance visualization of organelles in cultured cells, and selectively display single blood vessels in vivo in zebrafish larvae.