The reduced bactericidal activity of neutrophils as an incisive indicator of water-immersion restraint stress and impaired exercise performance in mice
The reduced bactericidal activity of neutrophils as an incisive indicator of water-immersion restraint stress and impaired exercise performance in mice
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DOI：
10.1038/s41598-019-41077-5
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发表时间：
2019-03-14
影响因子：
4.6
通讯作者：
Seki, Shuhji
中科院分区：
文献类型：
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作者：
Kinoshita, Manabu;Nakashima, Hiroyuki;Seki, Shuhji
对心理应激的深入评估可能是确定应激宿主运动表现的必要条件。我们研究了反映运动表现的心理应激的客观标志物，重点是中性粒细胞功能。我们使用小鼠水浸束缚（WIR）的压力，我们的评估。在接受WIR 1或2小时后，小鼠在气密跑步机上运动，监测其呼吸交换率。WIR应激后检测中性粒细胞功能。虽然对照小鼠（无WIR）在跑步机运动期间表现出良好的碳水化合物和脂质作为能量来源的燃烧，但经历2小时WIR的小鼠没有有效地燃烧碳水化合物或脂质，大大降低了它们的表现。相比之下，1小时WIR小鼠显示碳水化合物燃烧（尽管反应缓慢），但不使用脂质作为能量，因此比2小时WIR小鼠跑得更长，但比对照小鼠短。中性粒细胞的杀菌活性，但不是他们的超氧化物的生产或微球吞噬活性，显着减少1小时WIR和进一步减少2小时WIR，表明WIR应激和运动性能之间的显着关联。中性粒细胞杀菌活性可能是心理应激的一个很好的指标，也是精确评估运动能力的一个有用的工具。
The incisive evaluation of psychological stress may be required to determine the exercise performance of stressed hosts. We investigated objective markers of psychological stress that reflect exercise performance, focusing on the neutrophil function. We used murine water-immersion restraint (WIR) stress for our assessments. After receiving WIR for 1 or 2 h, mice were exercised on an airtight treadmill that monitors their respiratory exchange ratio. The neutrophil function was analyzed after WIR stress. Although the control mice (without WIR) showed good combustion of both carbohydrates and lipids as energy sources during treadmill exercise, mice that underwent 2-h WIR did not combust carbohydrates or lipids effectively, drastically reducing their performance. In contrast, the 1-h WIR mice showed carbohydrate combustion (albeit a slow response) but did not use lipids for energy, thereby running longer than the 2-h WIR mice but shorter than the control mice. The bactericidal activity of neutrophils, but not their superoxide production or microsphere-phagocytic activity, was significantly reduced by 1-h WIR and further reduced by 2-h WIR, indicating a significant association between WIR stress and exercise performance. The neutrophil bactericidal activity may be a good indicator of psychological stress and a useful tool for precisely assessing exercise performance.