Structure and function of the pancreas in the polycystic kidney rat
Structure and function of the pancreas in the polycystic kidney rat
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多囊肾大鼠胰腺的结构和功能
DOI：
10.1097/mpa.0b013e31824c12f9
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发表时间：
2012
期刊：
影响因子：
2.9
通讯作者：
Ishiguro H
中科院分区：
文献类型：
--
作者：
Yi L;Naruse S;Furuya S;Yamamoto A;Nakakuki M;Nagao S;Yoshihara D;Ko SB;Wei M;Kondo T;Ishiguro H
目的编码纤毛蛋白纤维囊肿蛋白的 Pkhd1 基因突变会导致多囊肾 (PCK) 大鼠（人类常染色体隐性遗传 PCK 疾病模型）的肾脏和肝脏出现多个囊肿。为了明确初级纤毛在胰管中的作用，我们对PCK大鼠的外分泌胰腺的结构和功能进行了检测。方法收集麻醉大鼠的胰液和胆汁。采用显微解剖和免疫组织化学方法对胰管结构进行分析。结果组织学上胰腺腺泡基本正常，胰腺内未发现囊肿。较大的胰管不规则扩张，上皮细胞中 AQP1 表达增强。在生理管腔压力（3 cm H 2 O）下，PCK大鼠的胰管表现出显着（P<0.05）比野生型（WT）大鼠更高的扩张性。用生理剂量的促胰液素（0.03 nmol/kg/小时）刺激PCK大鼠的胰液分泌显着小于WT，但在较高剂量下差异不显着。 PCK 和 WT 大鼠对氨甲酰胆碱的淀粉酶反应没有差异。结论这些发现表明纤维囊肿素/初级纤毛依赖性机制可能在胰腺导管结构和液体分泌的调节中发挥作用。
ObjectivesMutation in the Pkhd1 gene that encodes a ciliary protein, fibrocystin, causes multiple cysts in the kidneys and liver in the polycystic kidney (PCK) rat, a model for human autosomal recessive PCK disease. To clarify the role of primary cilia in the pancreatic duct, we examined the structure and function of the exocrine pancreas of PCK rats.MethodsPancreatic juice and bile were collected from anesthetized rats. Pancreatic ductal structure was analyzed by microdissection and immunohistchemistry.ResultsHistologically pancreatic acini were apparently normal, and no cysts were detected in the pancreas. Larger pancreatic ducts were irregularly dilated with enhanced expression of AQP1 in epithelial cells. The pancreatic duct of PCK rats exhibited significantly (P< 0.05) higher distensibility than that of wild-type (WT) rat at a physiological luminal pressure (3 cm H 2 O). Pancreatic fluid secretion stimulated with a physiological dose of secretin (0.03 nmol/kg per hour) in PCK rats was significantly smaller than that in WT, but the differences were not significant at higher doses. The amylase responses to carbamylcholine were not different between PCK and WT rats.ConclusionsThese findings suggest that fibrocystin/primary cilia–dependent mechanisms may play a role in the regulation of pancreatic ductal structure and fluid secretion.