A POTASSIUM CHANNEL MUTATION IN WEAVER MICE IMPLICATES MEMBRANE EXCITABILITY IN GRANULE CELL-DIFFERENTIATION
A POTASSIUM CHANNEL MUTATION IN WEAVER MICE IMPLICATES MEMBRANE EXCITABILITY IN GRANULE CELL-DIFFERENTIATION
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DOI：
10.1038/ng1095-126
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发表时间：
1995-10-01
期刊：
影响因子：
30.8
通讯作者：
PETERSON, AS
中科院分区：
文献类型：
--
作者：
PATIL, N;COX, DR;PETERSON, AS
神经元分化的早期事件通常被认为是由独立于膜通透性改变的因素调节的。Weaver小鼠体内存在一种突变，在细胞迁移和突触形成之前，细胞分裂停止后，这种突变会阻止神经元分化。纯合织布鼠的小脑颗粒细胞无法分化，要么是因为缺乏内在线索，要么是因为颗粒细胞无法对这些线索做出反应。我们现在报告织布鼠在编码G蛋白偶联的内向整流钾通道的基因中存在错义突变。突变改变了蛋白质的离子渗透性和孔区，这表明颗粒细胞的分化是由膜通透性的变化调节的。
Early events in neuronal differentiation are generally considered to be regulated by factors independent of alterations in membrane permeability. Weaver mice harbour a mutation that blocks neuronal differentiation just after cessation of cell division, prior to cell migration and synaptogenesis. Cerebellar granule cells in homozygous weaver mice fail to differentiate, either because intrinsic cues are absent or because the granule cells are unable to respond to those cues. We now report that weaver mice have a missense mutation in a gene encoding a G-protein coupled inward rectifier potassium channel. The mutation alters the putative ion-permeable, pore-forming domain of the protein, suggesting that granule cell differentiation is regulated by changes in membrane permeability.