Strategy for Rapid Detection of Carbapenemase-Producing Enterobacteriaceae
Strategy for Rapid Detection of Carbapenemase-Producing Enterobacteriaceae
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DOI：
10.1128/aac.01239-13
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发表时间：
2014-04-01
影响因子：
4.9
通讯作者：
Nordmann, Patrice
中科院分区：
文献类型：
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作者：
Dortet, Laurent;Brechard, Ludivine;Nordmann, Patrice
对862株对碳青霉烯类抗生素敏感性降低的肠杆菌科细菌进行了前瞻性调查。碳青霉烯酶产生菌的检测采用碳青霉烯酶显色法、紫外分光光度法和DNA微阵列技术，并与聚合酶链式反应和测序结果进行比较。用Carba NP试验和紫外分光光度法检测了172株碳青霉烯酶产生菌，而DNA微阵列未能检测到IMI产生菌。使用Carba NP试验作为第一次筛选，然后使用分子技术，已被确定为鉴定产生碳青霉烯酶的肠杆菌科细菌的有效策略。
A prospective survey was conducted on 862 Enterobacteriaceae isolates with reduced susceptibility to carbapenems. The Carba NP test, UV spectrophotometry, and a DNA microarray were used to detect carbapenemase producers, and the results were compared to those from PCR and sequencing. The 172 carbapenemase producers were detected using the Carba NP test and UV spectrophotometry, whereas the DNA microarray failed to detect IMI producers. The use of the Carba NP test as a first screening, followed by the use of molecular techniques, has been determined to be an efficient strategy for identifying carbapenemase-producing Enterobacteriaceae.