Sulforaphane suppressed LPS-induced inflammation in mouse peritoneal macrophages through Nrf2 dependent pathway.
Sulforaphane suppressed LPS-induced inflammation in mouse peritoneal macrophages through Nrf2 dependent pathway.
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DOI：
10.1016/j.bcp.2008.07.036
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发表时间：
2008-10-15
影响因子：
5.8
通讯作者：
Kong, Ah-Ng
中科院分区：
文献类型：
--
作者：
Lin, Wen;Wu, Rachel T.;Wu, Tienyuan;Khor, Tin-Oo;Wang, Hu;Kong, Ah-Ng
关键词：
萝卜硫素(SFN)是一种天然异硫氰酸酯，存在于花椰菜和卷心菜等十字花科蔬菜中。以往的研究表明，三七总皂苷能有效地预防致癌物对啮齿动物的致癌作用，这部分与其强大的抗炎特性有关。在本研究中，我们比较了三七总皂苷对内毒素刺激的Nrf2(+/+)和Nrf2小鼠的原代腹膜巨噬细胞炎症的抗炎作用。SFN对NRF2(+/+)原代培养的巨噬细胞有明显的抑制作用，可明显抑制脂多糖刺激的巨噬细胞α、IL-1、COX-2和β的表达和表达。脂多糖刺激的Nrf2(+/+)原代巨噬细胞HO-1表达明显增强。有趣的是，NRF2(−/−)原代腹膜巨噬细胞的抗炎作用减弱。结论：三七总皂苷的抗炎作用主要是通过激活小鼠腹腔巨噬细胞中的NRF2来实现的。
Sulforaphane (SFN) is a natural isothiocyanate that is present in cruciferous vegetables such as broccoli and cabbage. Previous studies have shown that SFN is effective in preventing carcinogenesis induced by carcinogens in rodents, which is related in part to its potent anti-inflammation properties. In the present study, we compared the anti-inflammatory effect of SFN on LPS-stimulated inflammation in primary peritoneal macrophages derived from Nrf2 (+/+) and Nrf2 mice. Pretreatment of SFN in Nrf2 (+/+) primary peritoneal macrophages potently inhibited LPS-stimulated mRNA expression, protein expression and production of TNFα, IL-1β, Cox-2 and iNOS. HO-1 expression, which is significantly augmented in LPS-stimulated Nrf2 (+/+) primary peritoneal macrophages by SFN. Interestingly, the anti-inflammatory effect was attenuated in Nrf2 (−/−) primary peritoneal macrophages. We concluded that SFN exerts its anti-inflammatory activity mainly via activation of Nrf2 in mouse peritoneal macrophages.
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影响因子：
11.2
作者：
Choi, S;Singh, SV
通讯作者：
Singh, SV
DOI：
10.1073/pnas.051632198
发表时间：
2001-03-13
影响因子：
11.1
作者：
Dinkova-Kostova, AT;Massiah, MA;Talalay, P
通讯作者：
Talalay, P
影响因子：
11.2
作者：
Shen, Guoxiang;Khor, Tin Oo;Kong, Ah-Ng Tony
通讯作者：
Kong, Ah-Ng Tony
影响因子：
4.7
作者：
Hu, Rong;Khor, Tin Oo;Kong, Ah-Ng Tony
通讯作者：
Kong, Ah-Ng Tony
影响因子：
4.6
作者：
Zhu, M;Zhang, YS;Bowden, GT
通讯作者：
Bowden, GT