Small intestinal mucins are secreted in proportion to the settling volume in water of dietary indigestible components in rats.
Small intestinal mucins are secreted in proportion to the settling volume in water of dietary indigestible components in rats.
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小肠粘蛋白的分泌与大鼠膳食中难消化成分在水中的沉降体积成比例。
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
T. Morita
中科院分区：
文献类型：
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作者：
H. Tanabe;K. Sugiyama;T. Matsuda;S. Kiriyama;T. Morita
本研究的目的是阐明膳食难消化成分对小肠粘蛋白分泌的影响。我们制备了具有不同膨胀比（PSF-30、-60 和 -90）的聚苯乙烯泡沫（PSF），其中粉末在水中具有不同的沉降体积（SV）。给大鼠喂食含有 0、10、30 或 90 g PSF-60/kg 的纯化饮食，持续 10 天。禁食 8 小时后，在 90 分钟内给大鼠喂食 3 克各自的饮食。制备小肠粘蛋白级分，并确定高碘酸/席夫反应物质和O-连接寡糖链作为粘蛋白标记物。饲喂 PSF-60 剂量依赖性地增加小肠粘蛋白分泌（对照与 30 或 60 g PSF-60/kg，P < 0.05）。当大鼠饲喂纯化饮食或含有 10 g/kg PSF-30、60 或 90 的饮食 7 天时，小肠粘蛋白受到相应测试 PSF SV 的极大影响。饲喂含有最高 SV 的 PSF-90 饮食的大鼠粘蛋白含量最高（与对照相比，P < 0.05）。在一些天然膳食纤维中，小肠粘蛋白和SV呈相关性（r = 0.967，P = 0.002）。最后，给大鼠喂食纯化饮食或含有50 g PSF-60/kg的饮食7天。然后，每个饮食组进一步分为2组。禁食 8 小时后，给大鼠喂食 3 g 纯化饮食或 PSF 饮食。小肠中的大量粘蛋白仅在先前饲喂 PSF 饮食的大鼠中出现，无论它们是重新饲喂纯化的还是 PSF 饮食（对照与 PSF，P < 0.05）。这些结果表明，小肠粘蛋白的分泌与膳食中难消化成分的SV成比例，并且需要长期摄入难消化成分才能出现粘蛋白分泌增强的现象。
The aim of this study was to clarify the effect of dietary indigestible components on small intestinal mucin secretion. We prepared polystyrene foam (PSF) with different expansion ratios (PSF-30, -60 and -90) in which powders had different settling volumes in water (SV). Rats were fed a purified diet containing 0, 10, 30, or 90 g of PSF-60/kg for 10 d. After 8 h of food deprivation, rats were refed 3 g of their respective diets within 90 min. Small intestinal mucin fractions were prepared, and periodic acid/Schiff-reactive substances and O-linked oligosaccharide chains were determined as mucin markers. Feeding of PSF-60 increased the small intestinal mucin secretion dose dependently (control vs. 30 or 60 g of PSF-60/kg, P < 0.05). When rats were fed either purified diet or diets containing PSF-30, 60, or 90 at 10 g/kg for 7 d, small intestinal mucins were greatly affected by the SV of the respective PSF tested. Rats fed the diet containing PSF-90 with the highest SV had the highest amount of mucins (vs. control, P < 0.05). In some natural dietary fibers, the small intestinal mucins and SV were correlated (r = 0.967, P = 0.002). Finally, rats were fed a purified diet or that diet containing 50 g of PSF-60/kg for 7 d. Then, each dietary group was further divided into 2 groups. After 8 h of food deprivation, rats were refed 3 g of purified or PSF diet. Greater mucins in the small intestine were manifest only in rats previously fed the PSF diet whether they were refed purified or PSF diet (control vs. PSF, P < 0.05). These results suggest that the small intestinal mucins are secreted in proportion to the SV of dietary indigestible components, and chronic ingestion of indigestible components is required for the appearance of enhanced mucin secretion.