Synthesis and transport characterization of alginate/aminopropylsilicate/alginate microcapsule: application to bioartificial pancreas
Synthesis and transport characterization of alginate/aminopropylsilicate/alginate microcapsule: application to bioartificial pancreas
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DOI：
10.1016/s0142-9612(01)00016-3
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发表时间：
2001-11-01
期刊：
影响因子：
14
通讯作者：
Kawakami, K
中科院分区：
文献类型：
--
作者：
Sakai, S;Ono, T;Kawakami, K
为了研制一种新型的免疫隔离膜用于微囊型生物人工胰腺，我们尝试使用溶胶-凝胶法合成硅酸盐。以海藻酸钙凝胶为载体，通过静电作用，制备了氨基丙基三甲氧基硅烷和四甲氧基硅烷的氨基丙基硅酸盐膜。通过浸入海藻酸钠水溶液中来中和残留在所得凝胶珠表面上的带正电的氨基。从不同物质对凝胶珠的分配系数和有效扩散率的测量，发现在硅醇盐前体的优化组成下制备的氨基丙基硅酸盐膜成功地排斥了γ-球蛋白，对具有低分子量的物质具有良好的渗透性。胰岛可以被封装在新开发的微胶囊中，同时保留其分泌胰岛素的能力。(C)2001爱思唯尔科技有限公司版权所有。
To develop a novel type of immunoisolation membrane for a microcapsule-shaped bioartificial pancreas, we attempted to use a sol-gel synthesized silicate. An aminopropyl-silicate membrane derived from 3-aminopropyltrimethoxysilane and tetramethoxysilane was formed on Ca-alginate gel beads via electrostatic interaction. The positively charged amino groups remaining on the surface of the resultant gel beads were neutralized by immersion in an aqueous Na-alginate solution. From measurements of the partition coefficients and effective diffusivities of different substances to the gel beads, it was found that the aminopropyl-silicate membrane prepared under optimized composition of silicon alkoxide precursors successfully rejected gamma -globulin, giving good permeability to substances having a low molecular weight. Islets could be encapsulated within the newly developed microcapsules while retaining their ability to secrete insulin. (C) 2001 Elsevier Science Ltd. All rights reserved.