Evaluation of Swabs, Transport Media, and Specimen Transport Conditions for Optimal Detection of Viruses by PCR
Evaluation of Swabs, Transport Media, and Specimen Transport Conditions for Optimal Detection of Viruses by PCR
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DOI：
10.1128/jcm.06551-11
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发表时间：
2012-03-01
影响因子：
9.4
通讯作者：
Birch, Chris
中科院分区：
文献类型：
--
作者：
Druce, Julian;Garcia, Katherine;Birch, Chris
流行病期间拭子和病毒运输介质的耗尽可能会促使使用未经验证的替代品。将干燥或盐水中收集和运输的拭子与市售拭子/培养基组合进行比较，用于流感、肠道病毒、单纯疱疹病毒和腺病毒的 PCR 检测。每个都在环境温度（22 摄氏度）和 4 摄氏度下检测 7 天。用干拭子或盐水拭子检测流感很重要，因为它能够引起涉及大量病例的疫情爆发。
Depletion of swabs and viral transport medium during epidemics may prompt the use of unvalidated alternatives. Swabs collected and transported dry or in saline were compared to commercially available swab/medium combinations for PCR detection of influenza, enterovirus, herpes simplex virus, and adenovirus. Each was detected at an ambient temperature (22 degrees C) and 4 degrees C for 7 days. Detection of influenza on dry or saline swabs is important because of its capacity to cause outbreaks involving large numbers of cases.