Identification of novel bone morphogenetic protein- responsive elements in a hepcidin promoter
Identification of novel bone morphogenetic protein- responsive elements in a hepcidin promoter
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铁调素启动子中新型骨形态发生蛋白反应元件的鉴定
DOI：
10.1002/1873-3468.12900
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发表时间：
2017
期刊：
影响因子：
3.5
通讯作者：
Funaba Masayuki
中科院分区：
文献类型：
--
作者：
Kanamori Yohei;Murakami Masaru;Matsui Tohru;Funaba Masayuki
海普西丁在全身铁代谢中起着核心作用。骨形态发生蛋白(BMP)途径通过转录激活启动子上的BMP反应元件(RES)1和2来调节海普西丁的表达。以往的研究还表明，BMP途径通过启动子上富含GC的序列刺激其靶基因的转录。对海普西丁启动子富含GC序列的搜索表明，13个区域横跨远端(A-F)、中(G-I)和近端(J-M)区域，其中在B-D区发现的富含GC元件的突变显示出在存在BMP-Re1突变的情况下对ALK3(QD)表达的反应性降低，这表明BMP途径充分表达海普西丁所需的元件。
Hepcidin plays a central role in systemic iron metabolism. The bone morphogenetic protein (BMP) pathway regulates expression of hepcidin through transcriptional activationviaBMP‐responsive elements (REs) 1 and 2 on the promoter. Previous studies also revealed that the BMP pathway stimulates transcription of its target genesviaGC‐rich sequences on the promoter. A search for GC‐rich sequences on the hepcidin promoter indicated 13 regions across the distal (A to F), middle (G to I), and proximal (J to M) areas; among them, mutations of the GC‐rich element found in regions B to D exhibited decreased responsiveness to ALK3(QD) expression in the presence of BMP‐RE1 mutations, indicating necessity of the elements for full expression of hepcidin by the BMP pathway.