ACTIN-BINDING PROTEIN REQUIREMENT FOR CORTICAL STABILITY AND EFFICIENT LOCOMOTION
ACTIN-BINDING PROTEIN REQUIREMENT FOR CORTICAL STABILITY AND EFFICIENT LOCOMOTION
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DOI：
10.1126/science.1549777
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发表时间：
1992-01-17
期刊：
影响因子：
56.9
通讯作者：
STOSSEL, TP
中科院分区：
文献类型：
--
作者：
CUNNINGHAM, CC;GORLIN, JB;STOSSEL, TP
三个来自人类恶性黑色素瘤的无关肿瘤细胞系缺乏肌动蛋白结合蛋白(ABP)，ABP在体外将肌动蛋白细丝与质膜糖蛋白连接起来。ABP缺陷的细胞运动受损，质膜出现环状气泡。在其中一个细胞系中表达A.BP后，恢复了细胞的易位运动，减少了细胞膜的起泡。这些发现表明，ABP在体内具有稳定皮质肌动蛋白的功能，是有效的细胞运动所必需的。
Three unrelated tumor cell lines derived from human malignant melanomas lack actin-binding protein (ABP), which cross-links actin filaments in vitro and connects these filaments to plasma membrane glycoproteins. The ABP-deficient cells have impaired locomotion and display circumferential blebbing of the plasma membrane. Expression of A.BP in one of the lines after transfection restored translocational motility and reduced membrane blebbing. These findings establish that ABP functions to stabilize cortical actin in vivo and is required for efficient cell locomotion.