Geometry sensing by self-organized protein patterns
Geometry sensing by self-organized protein patterns
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DOI：
10.1073/pnas.1206953109
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发表时间：
2012-09-18
影响因子：
11.1
通讯作者：
Schwille, Petra
中科院分区：
文献类型：
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作者：
Schweizer, Jakob;Loose, Martin;Schwille, Petra
在活细胞中，蛋白质能够组织比其尺寸大得多的空间。作为回报，细胞内空间的变化可以影响生化反应，使细胞能够感知它们的大小和形状。尽管有可能重建蛋白质自组织只有几个纯化的组件，我们仍然缺乏知识的几何边界如何影响时空蛋白质模式。遵循最小系统方法，我们使用纯化的蛋白质和电泳图案化的膜来研究空间限制对Min系统（细菌胞质分裂的空间调节器）的自组织的影响。我们发现，新兴的蛋白质模式，甚至响应的横向，二维几何形状的膜，这样，在三维细胞，最小蛋白质波旅行沿着最长轴的膜补丁。这表明，对于空间感测，Min系统不需要封闭在三维隔室中。使用一个计算模型，我们定量分析了我们的实验结果，并确定了持续绑定MinE的膜要求的最小系统感测几何形状。我们的研究结果提供了深入了解几何约束和生化模式之间的相互作用出现的非线性反应扩散系统。
In the living cell, proteins are able to organize space much larger than their dimensions. In return, changes of intracellular space can influence biochemical reactions, allowing cells to sense their size and shape. Despite the possibility to reconstitute protein self-organization with only a few purified components, we still lack knowledge of how geometrical boundaries affect spatiotemporal protein patterns. Following a minimal systems approach, we used purified proteins and photolithographically patterned membranes to study the influence of spatial confinement on the self-organization of the Min system, a spatial regulator of bacterial cytokinesis, in vitro. We found that the emerging protein pattern responds even to the lateral, two-dimensional geometry of the membrane such that, as in the three-dimensional cell, Min protein waves travel along the longest axis of the membrane patch. This shows that for spatial sensing the Min system does not need to be enclosed in a three-dimensional compartment. Using a computational model we quantitatively analyzed our experimental findings and identified persistent binding of MinE to the membrane as requirement for the Min system to sense geometry. Our results give insight into the interplay between geometrical confinement and biochemical patterns emerging from a nonlinear reaction-diffusion system.