Comparative dietary sulfated metabolome analysis reveals unknown metabolic interactions of the gut microbiome and the human host
Comparative dietary sulfated metabolome analysis reveals unknown metabolic interactions of the gut microbiome and the human host
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DOI：
10.1016/j.freeradbiomed.2020.09.006
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发表时间：
2020-11-20
影响因子：
7.4
通讯作者：
Globisch, Daniel
中科院分区：
文献类型：
--
作者：
Correia, Mario S. P.;Jain, Abhishek;Globisch, Daniel
肠道微生物组转化膳食化合物，这些化合物在胃肠道中被吸收并进一步被人类宿主代谢。硫酸盐代谢物是来自这种共代谢的主要化合物类别，并且与疾病发展有关。在目前的多学科研究中，我们调查了来自饮食干预研究的人尿样，其中22人在食用富含多酚的早餐之前和之后收集。这些样品进行了分析，利用我们的方法相结合的酶代谢产物水解，使用芳基硫酸酯酶和质谱代谢组学。本研究的关键是验证235种结构不同的硫酸化代谢物。我们已经确定了48个显着上调代谢物的饮食干预，包括11个以前未知的硫酸代谢物，这种饮食。我们观察到受试者根据其硫酸盐代谢潜力存在较大差异，这可能是在未来大型队列研究中将受试者分类为高和低硫酸盐代谢者的基础。所报道的基于硫酸酯酶的方法是发现人体样品中未知微生物衍生代谢物的稳健工具。
The gut microbiome converts dietary compounds that are absorbed in the gastrointestinal tract and further metabolized by the human host. Sulfated metabolites are a major compound class derived from this co-metabolism and have been linked to disease development. In the present multidisciplinary study, we have investigated human urine samples from a dietary intervention study with 22 individuals collected before and after consumption of a polyphenol rich breakfast. These samples were analyzed utilizing our method combining enzymatic metabolite hydrolysis using an arylsulfatase and mass spectrometric metabolomics. Key to this study is the validation of 235 structurally diverse sulfated metabolites. We have identified 48 significantly upregulated metabolites upon dietary intervention including 11 previously unknown sulfated metabolites for this diet. We observed a large variation in subjects based on their potential to sulfate metabolites, which may be the foundation for classification of subjects as high and low sulfate metabolizers in future large cohort studies. The reported sulfatase-based method is a robust tool for the discovery of unknown microbiota-derived metabolites in human samples.