High-speed wide-field multi-parametric photoacoustic microscopy.
High-speed wide-field multi-parametric photoacoustic microscopy.
复制标题
DOI：
10.1364/ol.391824
复制
发表时间：
2020-05-15
期刊：
影响因子：
3.6
通讯作者：
Hu S
中科院分区：
文献类型：
--
作者：
Zhong F;Bao Y;Chen R;Zhou Q;Hu S
多参数光声显微镜(PAM)能够在微观水平上同时成像血液灌流、氧合和血流，是研究生理刺激或病理过程中血流动力学和代谢变化的有力工具。然而，基于相关性的血流测量的低扫描速度阻碍了其在快速微血管反应研究中的应用。为了应对这一挑战，我们开发了一种新的多参数PAM系统。通过使用圆柱形聚焦超声换能器(焦点区域：76μm×4.5 mm)扩展光学扫描范围以同时采集500个B超，新系统的速度比我们以前使用球形聚焦换能器(焦点直径：40μm)的多参数系统快112倍，并能够以1赫兹的帧速率在4.5x1mm2的区域内对血液灌流、氧合和血流进行高分辨率成像。我们已经在活体小鼠耳中验证了该系统的可行性。将该系统进一步发展为反射模式，将使小鼠大脑中血液动力学和新陈代谢的实时皮质成像成为可能。
Capable of imaging blood perfusion, oxygenation and flow simultaneously at the microscopic level, multi-parametric photoacoustic microscopy (PAM) has quickly emerged as a powerful tool for studying hemodynamic and metabolic changes due to physiological stimulations or pathological processes. However, the low scanning speed poised by the correlation-based blood flow measurement impedes its application in studying rapid microvascular responses. To address this challenge, we have developed a new multi-parametric PAM system. By extending the optical scanning range with a cylindrically focused ultrasonic transducer (focal zone: 76 μm × 4.5 mm) for simultaneous acquisition of 500 B-scans, the new system is 112 times faster than our previous multi-parametric system that uses a spherically focused transducer (focal diameter: 40 μm) and enables high-resolution imaging of blood perfusion, oxygenation and flow over an area of 4.5×1 mm2 at a frame rate of 1 Hz. We have demonstrated the feasibility of this system in the living mouse ear. Further development of this system into reflection mode will enable real-time cortex-wide imaging of hemodynamics and metabolism in the mouse brain.