Fine mapping and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.)
Fine mapping and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.)
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水稻光热敏性雄性不育基因ptgms2-1的精细定位及候选基因分析
DOI：
10.1007/s00122-010-1452-0
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发表时间：
2011-02-01
影响因子：
5.4
通讯作者：
Liang, Guohua
中科院分区：
文献类型：
--
作者：
Xu, Jianjun;Wang, Baohe;Liang, Guohua
光温敏核不育水稻具有许多优良的杂交水稻性状。克隆水稻光温敏核不育基因、阐明水稻雄性不育机理和育性可逆性的基因对培育新的不育系具有重要意义。广占63 S是我国应用最广泛的籼型两系杂交稻光温敏核不育系。本研究以广占63 S与1587杂交的F-2和BC 1F 2群体为材料，通过遗传分析，确定广占63 S的雄性不育性受单隐性基因控制。利用分子标记技术结合群体分离分析（BSA），将目的基因（ptgms 2 -1）定位在两个SSR标记RM 12521和RM 12823之间。利用在RM 12521和RM 12823区域之间开发的45个新的插入-缺失（InDel）标记，对来自F2和BC 1F 2群体的634个不育个体进行了ptgms 2 -1基因座的精细定位。Ptgms 2 -1进一步定位在两个InDel标记S2-40和S2-44之间的一个50.4kb的DNA片段上，遗传距离分别为0.08和0.16cM，与位于AP 004039 BAC克隆上的S2-43共分离。根据RiceGAAS系统的注释结果，在该区域鉴定出10个基因。核核糖核酸酶Z基因被鉴定为ptgms 2 -1基因的候选基因。这一结果将有助于ptgms 2 -1基因的克隆。ptgms 2 -1基因位点的紧密连锁标记将进一步为该基因的分子标记辅助选择提供有用的工具。
Photoperiod-thermo-sensitive genic male sterile (PTGMS) rice exhibits a number of desirable traits for hybrid rice production. The cloning genes responsible for PTGMS and those elucidating male sterility mechanisms and reversibility to fertility would be of great significance to provide a foundation to develop new male sterile lines. Guangzhan63S, a PTGMS line, is one of the most widely used indica two-line hybrid rice breeding systems in China. In this study, genetic analysis based on F-2 and BC1F2 populations derived from a cross between Guangzhan63S and 1587, determined a single recessive gene controls male sterility in Guangzhan63S. Molecular marker techniques combined with bulked-segregant analysis (BSA) were used and located the target gene (named ptgms2-1) between two SSR markers RM12521 and RM12823. Fine mapping of the ptgms2-1 locus was conducted with 45 new Insertion-Deletion (InDel) markers developed between the RM12521 and RM12823 region, using 634 sterile individuals from F-2 and BC1F2 populations. Ptgms2-1 was further mapped to a 50.4 kb DNA fragment between two InDel markers, S2-40 and S2-44, with genetic distances of 0.08 and 0.16 cM, respectively, which cosegregated with S2-43 located on the AP004039 BAC clone. Ten genes were identified in this region based on annotation results from the RiceGAAS system. A nuclear ribonuclease Z gene was identified as the candidate for the ptgms2-1 gene. This result will facilitate cloning the ptgms2-1 gene. The tightly linked markers for the ptgms2-1 gene locus will further provide a useful tool for marker-assisted selection of this gene in rice breeding programs.