A fetal human heart cardiac-inducing RNA (CIR) promotes the differentiation of stem cells into cardiomyocytes.
A fetal human heart cardiac-inducing RNA (CIR) promotes the differentiation of stem cells into cardiomyocytes.
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胎儿心脏诱导 RNA (CIR) 促进干细胞分化为心肌细胞。
DOI：
10.1007/s11626-015-9880-4
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Lemanski,LarryF
中科院分区：
文献类型：
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作者：
Kochegarov,Andrei;Moses-Arms,Ashley;Lemanski,LarryF
已经发现了一种特异性的人类胎儿心脏RNA，它具有诱导培养的小鼠胚胎和人类诱导的多能干细胞形成心肌细胞的能力。在这项研究中，从人类胎儿心脏中获得的商业RNA被克隆，测序，并使用标准的实验室方法合成。特异性胎心诱导RNA （CIR）的分子分析表明，它是n -磺基氨基磺酸水解酶和caspase募集结构域家族成员14前体的片段。经CIRs转染的干细胞常形成具有心肌细胞特征的梭形细胞，免疫组化染色检测，除了原肌球蛋白和α-肌动蛋白外，还表达心肌特异性收缩蛋白标志物肌钙蛋白- t。这些收缩蛋白的表达显示成肌纤维组织，具有心肌横纹肌细胞的特征。对活性CIR的RNA二级结构的计算机分析显示，它与蝾螈或肌原纤维诱导RNA （MIR）的RNA有显著的相似性，后者也能促进非肌肉细胞分化为心肌。因此，这两种rna，蝾螈MIR和新发现的人类克隆的CIR，似乎具有进化上保守的二级结构，这表明它们在脊椎动物心脏发育中起着重要作用，特别是在发育过程中心肌细胞与非肌肉细胞的分化中。
A specific human fetal heart RNA has been discovered, which has the ability to induce myocardial cell formation from mouse embryonic and human-induced pluripotent stem cells in culture. In this study, commercially obtained RNA from human fetal heart was cloned, sequenced, and synthesized using standard laboratory approaches. Molecular analyses of the specific fetal cardiac-inducing RNA (CIR), revealed that it is a fragment ofN-sulfoglucosaminesulfohydrolase and the caspase recruitment domain family member 14 precursor. Stem cells transfected with CIRs often form into spindle-shaped cells characteristic of cardiomyocytes,and express the cardiac-specific contractile protein marker, troponin-T, in addition to tropomyosin and α-actinin as detected by immunohistochemical staining. Expression of these contractile proteins showed organization into sarcomeric myofibrils characteristic of striated cardiac muscle cells. Computer analyses of the RNA secondary structures of the active CIR show significant similarities to a RNA from salamander or myofibril-inducing RNA (MIR), which also promotes non-muscle cells to differentiate into cardiac muscle. Thus, these two RNAs, salamander MIR and the newly discovered human-cloned CIR reported here, appear to have evolutionarily conserved secondary structures suggesting that both play major roles in vertebrate heart development and, particularly, in the differentiation of cardiomyocytes from non-muscle cells during development.