Propidium ion enters viable cells with high membrane potential during live-dead staining
Propidium ion enters viable cells with high membrane potential during live-dead staining
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DOI：
10.1016/j.mimet.2017.09.011
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发表时间：
2017-11-01
影响因子：
2.2
通讯作者：
Cypionka, Heribert
中科院分区：
文献类型：
--
作者：
Kirchhoff, Christian;Cypionka, Heribert
对膜电位高的细菌，用碘化丙啶染色可得出错误的结果。我们观察到shibae Dinoroseobacter和枯草芽孢杆菌（Bacillus subtilis）在完整细胞膜上摄取丙酸离子。显然，高膜电位有利于双荷丙离子的突破，并可以标志活细胞死亡。
Live-dead staining with propidium iodide can give erroneous results for bacteria showing high membrane potentials. We observed uptake of propidium ions across intact cell membranes for Dinoroseobacter shibae and Bacillus subtilis. Apparently, a high membrane potential facilitates breakthrough of the double-charged propidium ion and can mark viable cells as dead.