Prefrontal cortex, caloric restriction and stress during aging: Studies on dopamine and acetylcholine release, BDNF and working memory
Prefrontal cortex, caloric restriction and stress during aging: Studies on dopamine and acetylcholine release, BDNF and working memory
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DOI：
10.1016/j.bbr.2010.07.024
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发表时间：
2011-01-01
影响因子：
2.7
通讯作者：
Mora, Francisco
中科院分区：
文献类型：
--
作者：
Del Arco, Alberto;Segovia, Gregorio;Mora, Francisco
本研究旨在调查大鼠一生中长期热量限制是否会改变急性轻度应激对前额叶皮层 (PFC) 多巴胺和乙酰胆碱释放以及工作记忆表现的影响。还监测了自发运动活动，并测量了前额皮质、杏仁核和海马体的 BDNF 水平。将雄性 Wistar 大鼠（3 个月大）在热量限制（CR；40% 食物摄入限制）或对照条件下圈养 3、12、21 和 27 个月（圈养结束时为 6、15、24 和 30 个月）。经过行为测试后，动物被进一步分为另外两组。在其中一组中测定了 BDNF 蛋白水平。另一组大鼠将引导插管植入前额皮质进行微透析实验。 CR 大鼠因处理应激而释放的多巴胺与 6、15 和 24 月龄对照大鼠的反应没有差异。乙酰胆碱的释放在6个月和15个月时没有变化，但在24个月时减少。压力不会改变 30 月龄 CR 和对照大鼠的多巴胺或乙酰胆碱释放。 6 个月和 15 个月 CR 大鼠的海马和杏仁核中 BDNF 水平增加，但 PFC 中没有增加。所有组 CR 大鼠的自发运动活动均有所增加。然而，年龄会降低 CR 和对照大鼠的运动活动。两个实验组在基础条件下和急性应激情况下的延迟交替任务中都表现出相似的工作记忆表现。这些结果表明，CR 不会改变 PFC 响应急性应激的功能，也不会改变正常衰老过程中发现的变化。 (C) 2010 Elsevier B.V. 保留所有权利。
This study was designed to investigate whether long-term caloric restriction during the life span of the rat changes the effects of an acute mild stress on the release of dopamine and acetylcholine in the prefrontal cortex (PFC) and on working memory performance. Spontaneous motor activity was also monitored and levels of BDNF measured in the prefrontal cortex, amygdala and hippocampus. Male Wistar rats (3 months of age) were housed during 3, 12,21 and 27 months (6, 15,24 and 30 months of age at the end of housing) in caloric restriction (CR; 40% food intake restriction) or control conditions. After behavioural testing, animals were further subdivided into two other groups. In one of the groups BDNF protein levels were determined. In the other group rats were implanted with guide cannulas into the PFC to perform microdialysis experiments. In CR rats the release of dopamine produced by handling stress did not differ from the response found in control rats of 6, 15 and 24 months of age. The release of acetylcholine was not changed at the ages of 6 and 15 months but reduced at the age of 24 months. Stress did not change dopamine or acetylcholine release in CR and control rats of 30 months of age. BDNF levels were increased in the hippocampus and amygdala, but not in the PFC, of 6 and 15 months CR rats. Spontaneous motor activity was increased in all groups of CR rats. Age, however, decreased motor activity in CR and control rats. Both experimental groups showed similar working memory performance in a delayed alternation task in basal conditions and after a situation of acute stress. These results suggest that CR does not modify the function of the PFC in response to an acute stress nor the changes found as a result of the normal process of aging. (C) 2010 Elsevier B.V. All rights reserved.