Steroid formation and differentiation of cortical cells in tissue culture of human fetal adrenals in the presence and absence of ACTH.
Steroid formation and differentiation of cortical cells in tissue culture of human fetal adrenals in the presence and absence of ACTH.
复制标题
在存在和不存在 ACTH 的情况下，人胎儿肾上腺组织培养中皮质细胞的类固醇形成和分化。
DOI：
10.1210/endo-98-1-33
复制
发表时间：
1976
期刊：
影响因子：
4.8
通讯作者：
M. Salmenperä
中科院分区：
文献类型：
--
作者：
A. Kahri;I. Huhtaniemi;M. Salmenperä
观察带ACTH和不带ACTH的人胎儿肾上腺皮质细胞组织培养中类固醇分泌和超微结构分化情况。采用气相液相色谱和气相色谱-质谱联用技术对未偶联和磺化内源性中性类固醇进行分析。中性类固醇的胎儿模式，包括高浓度的硫酸盐偶联物，在培养的前五天被发现。在培养6至11天，这些类固醇的浓度下降。然而，当ACTH刺激时，在第6天至第11天，类固醇分泌量增加，其模式转变为成人型，皮质醇分泌率提高30-200倍。培养中能够增殖的皮质细胞具有胎儿肾上腺型和成人肾小球带型永久带细胞的超微结构。ACTH刺激诱导这些细胞分化为束状带型。提示ACTH是成人肾上腺皮质发育的主要激素调节因子，胎儿期存在抑制3β -羟基类固醇脱氢酶系统合成的因子。
Steroid secretion and ultrastructural differentiation of human fetal adrenal cortical cells were analyzed in tissue culture with and without ACTH. The unconjugated and sulfated endogenous neutral steroids were analyzed by gas-liquid chromatography and gas chromatography-mass spectrometry. A fetal pattern of neutral steroids, including high concentrations of sulfate conjugates, was found during the first five days of the cultivation. At 6 to 11 days of cultivation, a decrease was seen in concentrations of these steroids. However, when stimulated with ACTH, an increasing amount of steroids was secreted during days 6 to 11 and their pattern was transformed into the adult type with a 30-200 times higher secretion rate of cortisol. Cortical cells capable of proliferation in the culture had the ultrastructure of the permanent zone cells of the fetal adrenal or adult zona glomerulosa type. ACTH stimulation induced a differentiation of these cells into zona fasciculata type. The results suggest that ACTH is the main hormonal regulator in the genesis of the adult human adrenal cortex and that there is a factor during fetal life which inhibits the synthesis of the 3beta-hydroxysteroid dehydrogenase system.