Preparation of core-shell Fe3O4@poly(dopamine) magnetic nanoparticles for biosensor construction
Preparation of core-shell Fe3O4@poly(dopamine) magnetic nanoparticles for biosensor construction
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DOI：
10.1039/c3tb21171a
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发表时间：
2014-01-01
影响因子：
7
通讯作者：
Luis Gonzalez-Mora, Jose
中科院分区：
文献类型：
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作者：
Martin, Miriam;Salazar, Pedro;Luis Gonzalez-Mora, Jose
采用原位自聚合法制备了新型核壳型Fe3O4@poly（多巴胺）磁性纳米颗粒。该杂化纳米材料的平均芯径为11 +/- 3 nm，聚合物薄膜厚度为1.8 +/- 0.2 nm。将核壳纳米粒子作为固体载体，用于辣根过氧化物酶（HRP）的共价固定化，并利用所获得的生物功能化磁性纳米粒子构建H2O2的安培生物传感器。该酶生物传感器灵敏度为442.14 mA M-1 cm(-2)，低检出限为182 nM，线性范围为6.0 × 10(-7) ~ 8.0 × 10(-4) M，稳定性为1个月。
Novel core-shell Fe3O4@poly(dopamine) magnetic nanoparticles were prepared through an in situ self-polymerization method. The hybrid nanomaterial showed an average core diameter of 11 +/- 3 nm and a polymer thin film thickness of 1.8 +/- 0.2 nm. The core-shell nanoparticles were employed as solid supports for the covalent immobilization of horseradish peroxidase (HRP), and the resulting biofunctionalized magnetic nanoparticles were employed to construct an amperometric biosensor for H2O2. The enzyme biosensor showed a high sensitivity of 442.14 mA M-1 cm(-2), a low limit of detection of 182 nM, a wide linear range from 6.0 x 10(-7) to 8.0 x 10(-4) M and high stability for 1 month.