POLYAMINE INDUCED AGGREGATION OF DNA
POLYAMINE INDUCED AGGREGATION OF DNA
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DOI：
10.1093/nar/4.3.685
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发表时间：
1977-01-01
影响因子：
14.9
通讯作者：
KLEPPE, K
中科院分区：
文献类型：
--
作者：
OSLAND, A;KLEPPE, K
多在线诱导的各种DNA聚集已经研究了在条件下通常采用在许多酶的分析，其中DNA是底物之一。到目前为止，精胺是最有效的多胺，其次是亚精胺和尸胺。发现所有双链和自然产生的单链hcta都聚集。在相同的条件下，没有检测到单链聚氧聚合物的聚集。聚合开始时的多胺浓度被发现是DNA浓度的线性函数。曲线的斜率取决于多胺的性质、DNA、Mg++浓度和离子强度。
Poly-online induced aggregation of various DNAs has been studied under conditions usually employed in many enzymatic assays where DNA is one of the substrates. Spermine was by far the most efficient polyamine in causing aggregation followed by spermidine and cadaverine. All double-stranded and naturally occurring single-stranded HCTAs were found to aggregate. No aggregation of single-stranded homodeoxypoly-mers could be detected under the same conditions. The concentration of polyamine at which the aggregation commenced was found to be a linear function of the DNA concentration. The slope of the curves depended on the nature of the polyamine, DNA the concentration of Mg++and the ionic strength.