Effect of Peroxisome Proliferator-Activated Receptor Gamma Agonists on Myofibroblast Differentiation and Collagen Production in Nasal Polyp-Derived Fibroblasts
Effect of Peroxisome Proliferator-Activated Receptor Gamma Agonists on Myofibroblast Differentiation and Collagen Production in Nasal Polyp-Derived Fibroblasts
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DOI：
10.1177/000348940911801007
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发表时间：
2009-10-01
影响因子：
1.4
通讯作者：
Cho, Jae Hoon
中科院分区：
文献类型：
--
作者：
Lee, Heung-Man;Kang, Hee Jun;Cho, Jae Hoon
目的：我们研究了转化生长因子-β 1（TGF-β 1）对鼻息肉来源的成纤维细胞（NPDF）分化及其胶原蛋白产生的影响，并研究了过氧化物酶体增殖物激活受体γ（PPARγ）激动剂对TGF-β 1的影响。方法：从5个人鼻息肉组织中建立原代成纤维细胞系。我们用 TGF-β 1 激活 NPDF，并将其暴露于 PPAR γ 激动剂环格列酮和 15-脱氧-Delta-前列腺素 J2。通过逆转录聚合酶链式反应、免疫细胞化学染色和胶原蛋白测定来测量α-平滑肌肌动蛋白（α-SMA）和胶原蛋白的表达。结果：TGF-β1处理增加了α-SMA和胶原蛋白的表达，而PPARγ激动剂显着减弱了表达。结论：PPARγ激动剂抑制TGF-β1激活的NPDF的分化及其胶原蛋白的产生。
Objectives: We investigated the effect of transforming growth factor-beta 1 (TGF-beta 1) on the differentiation of nasal polyp-derived Fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor gamma (PPAR gamma) agonists on the effects of TGF-beta 1.Methods: Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-beta 1 and exposed them to the PPAR gamma agonists ciglitazone and 15-deoxy-Delta-prostaglandin J2. The expression of alpha-smooth muscle actin (alpha-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays.Results: The treatment with TGF-beta 1 increased the expression of alpha-SMA and collagen, and expression was markedly attenuated by the PPAR gamma agonists.Conclusions: PPAR gamma agonists inhibit the differentiation of TGF-beta 1-activated NPDFs and their production of collagen.