EFFECTS OF STRUCTURAL FEATURES OF COTTON CELLULOSE ON ENZYMATIC-HYDROLYSIS
EFFECTS OF STRUCTURAL FEATURES OF COTTON CELLULOSE ON ENZYMATIC-HYDROLYSIS
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DOI：
10.1002/app.1981.070260622
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发表时间：
1981-01-01
影响因子：
3
通讯作者：
CARNITI, P
中科院分区：
文献类型：
--
作者：
FOCHER, B;MARZETTI, A;CARNITI, P
用γ射线和18% NaOH和70% zncl2溶液处理纺织棉废料，并进行酶解。通过分子结构（聚合度）、超分子结构（x射线衍射）、可及性和反应性（回潮率、酶吸附和在fena中的溶解度）等参数，对未处理和处理过的样品在水解前后进行了表征。这些参数与水解反应的动力学参数相关。利用Lineweaver-Burk图计算不同温度下的vmaxand kmvalue。vmax /Kmratio，类似于特异性常数，被证明对实验误差不太敏感，更适合于比较样品的动力学行为。纤维素的超分子结构和形态的修饰是获得高产率和水解率的主要因素。此外，用于表征样品的结构和形态参数可以与酶解的动力学参数相关，特别是kkm值。
Textile cotton wastes were treated with γ rays and 18% NaOH and 70% ZnCl2solutions and were subjected to enzymatic hydrolysis. The untreated and treated samples were characterized both before and after hydrolysis by means of parameters concerning molecular structure (degree of polymerization), supermolecular structure (x‐ray diffraction), accessibility, and reactivity (moisture regain, enzyme adsorption, and solubility in FeTNa). These parameters were correlated to kinetic parameters of the hydrolysis reaction. TheVmaxandKmvalues were evaluated from Lineweaver–Burk plots at different temperatures. TheVmax/Kmratio, analogous to the specificity constant, proved to be less sensitive to experimental errors and more suitable for a comparison of the kinetic behavior of the samples. The modifications of both supermolecular structure and morphology of cellulose were of primary importance to attain high yields and rates of hydrolysis. Furthermore, the structural and morphologic parameters chosen to characterize the samples can be correlated to the kinetic parameters of enzymatic hydrolysis, in particular toKmvalues.