Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development
Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development
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DOI：
10.1631/jzus.b1400086
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发表时间：
2014-09-01
影响因子：
5.1
通讯作者：
Yu, Ming-liang
中科院分区：
文献类型：
--
作者：
Jiao, Yun;Ma, Rui-juan;Yu, Ming-liang
血肉桃因其诱人的花青素诱导的色素沉着和抗氧化特性而在中国很受欢迎。在这项研究中，我们通过检测桃中果皮中花青素生物合成途径的九个基因的表达来研究花青素积累的分子机制。利用定量逆转录聚合酶链式反应(qRT-PCR)技术，测定了2个血肉桃品种和1个白肉桃品种果实在6个发育阶段的表达。结果表明，查尔酮合成酶(CHS)基因的表达与两种血肉桃中花青素的积累密切相关。在白肉桃果实发育过程中，尽管花青素生物合成途径的其他基因均高表达，但苯丙氨酸解氨酶(PAL)的转录水平明显低于血肉桃，这表明PAL基因可能限制了白肉桃花青素的合成。此外，在去袋后3天，血肉桃中CHS和UDP-葡萄糖-黄酮类3-O-葡萄糖基转移酶(UFGT)基因的转录水平显著上调，这表明CHS和UFGT基因是两个血肉桃花青素生物合成过程中的关键基因。本研究将有助于更好地理解血肉桃花青苷积累的分子机制。
The blood-flesh peach has become popular in China due to its attractive anthocyanin-induced pigmentation and antioxidant properties. In this study, we investigated the molecular mechanisms underlying anthocyanin accumulation by examining the expression of nine genes of the anthocyanin biosynthesis pathway found in the peach mesocarp. Expression was measured at six developmental stages in fruit of two blood-flesh and one white-flesh peach cultivars, using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results show that the expression of the chalcone synthase (CHS) gene was closely related to anthocyanin accumulation in both of the blood-flesh peaches. In the white-flesh peach, we found that the transcription level of phenylalanine ammonia-lyase (PAL) during fruit development was much lower than that in the blood-flesh peach, even though all other genes of the anthocyanin biosynthesis pathway were highly expressed, suggesting that the PAL gene may be limiting in anthocyanin production in the white-flesh peach. Moreover, the transcription levels of the CHS and UDP-glucose-flavonoid 3-O-glucosyltransferase (UFGT) genes were markedly up-regulated at three days after bag removal (DABR) in the blood-flesh peach, suggesting that CHS and UFGT are the key genes in the process of anthocyanin biosynthesis for both of the blood-flesh peaches. The present study will be of great help in improving understanding of the molecular mechanisms involved in anthocyanin accumulation in blood-flesh peaches.