Electrospun silk-BMP-2 scaffolds for bone tissue engineering
Electrospun silk-BMP-2 scaffolds for bone tissue engineering
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DOI：
10.1016/j.biomaterials.2006.01.022
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发表时间：
2006-06-01
期刊：
影响因子：
14
通讯作者：
Kaplan, DL
中科院分区：
文献类型：
--
作者：
Li, CM;Vepari, C;Kaplan, DL
通过静电纺丝制备含有骨形态发生蛋白2 （BMP-2）和/或羟基磷灰石纳米颗粒（nHAP）的丝素纤维支架，用于人骨髓间充质干细胞（hMSCs）的体外成骨。BMP-2以生物活性形式在水基电纺丝过程中存活下来。在成骨培养基中，将hMSCs在支架（蚕丝/PEO/BMP-2、蚕丝/PEO/nHAP、蚕丝/PEO/nHAP/BMP-2）和对照（蚕丝/PEO、蚕丝/PEO萃取物）上静培养31天。基于电纺丝蛋白的支架支持hMSC生长和向成骨结果分化。与对照组相比，共处理BMP-2的支架支持更高的钙沉积和更高的骨特异性标志物转录水平，这表明这些纳米纤维静电纺丝支架是BMP-2的有效递送系统。x射线衍射（XRD）分析表明，在丝素蛋白/BMP-2支架上形成的磷灰石结晶度高于丝素蛋白支架对照组。此外，nHAP颗粒在加工过程中加入到电纺丝纤维支架中，并改善骨形成。与其他系统相比，电纺丝丝素纤维中BMP-2和nHAP共存导致钙沉积最多，BMP-2转录物水平上调。结果表明，基于静电纺丝的丝素蛋白支架是骨组织工程的潜在候选材料。此外，旋转纤维所需的温和水处理为将不稳定的细胞因子和其他成分输送到系统中提供了重要的选择。(c) 2006 Elsevier Ltd.版权所有。
Silk fibroin fiber scaffolds containing bone morphogenetic protein 2 (BMP-2) and/or nanoparticles of hydroxyapatite (nHAP) prepared via electrospinning were used for in vitro bone formation from human bone marrow-derived mesenchymal stem cells (hMSCs). BMP-2 survived the aqueous-based electrospinnig process in bioactive form. hMSCs were cultured for up to 31 days under static conditions in osteogenic media on the scaffolds (silk/PEO/BMP-2, silk/PEO/nHAP, silk/PEO/nHAP/BMP-2) and controls (silk/PEO, silk/PEO extracted). Electrospun silk fibroin-based scaffolds supported hMSC growth and differentiation toward osteogenic outcomes. The scaffolds with the co-processed BMP-2 supported higher calcium deposition and enhanced transcript levels of bone-specific markers than in the controls, indicating that these nanofibrous electrospun silk scaffolds were an efficient delivery system for BMP-2. X-ray diffraction (XRD) analysis revealed that the apatite formed on the silk fibroin/BMP-2 scaffolds had higher crystallinity than on the silk fibroin scaffold controls. In addition, nHAP particles were incorporated into the electrospun fibrous scaffolds during processing and improved bone formation. The coexistence of BMP-2 and nHAP in the electrospun silk fibroin fibers resulted in the highest calcium deposition and upregulation of BMP-2 transcript levels when compared with the other systems. The results suggest that electrospun silk-fibroin-based scaffolds are potential candidates for bone tissue engineering. Furthermore, the mild aqueous process required to spin the fibers offers an important option for delivery of labile cytokines and other components into the system. (c) 2006 Elsevier Ltd. All rights reserved.