Intron-Mediated Alternative Splicing of WOOD-ASSOCIATED NAC TRANSCRIPTION FACTOR1B Regulates Cell Wall Thickening during Fiber Development in Populus Species1[W]
Intron-Mediated Alternative Splicing of WOOD-ASSOCIATED NAC TRANSCRIPTION FACTOR1B Regulates Cell Wall Thickening during Fiber Development in Populus Species1[W]
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DOI：
10.1104/pp.113.231134
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发表时间：
2014-01
期刊：
影响因子：
7.4
通讯作者：
Yunjun Zhao;Jiayan Sun;Peng Xu;Rui Zhang;Laigeng Li
中科院分区：
文献类型：
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作者：
Yunjun Zhao;Jiayan Sun;Peng Xu;Rui Zhang;Laigeng Li
转录因子的选择性剪接产生两种异构体，它们在植物纤维细胞分化过程中调节细胞壁增厚中发挥拮抗作用。选择性剪接是调节多细胞生物发育的重要机制。尽管植物中的许多基因都经历选择性剪接，但人们对其在调控植物生长发育中的意义知之甚少。在这项研究中，选择性剪接的黑棉白杨（杨）木材相关的NAC结构域转录因子（PtrWNDs），PtrWND 1B，只发生在次生木质部纤维细胞。PtrWND 1B与正常的短转录本PtrWND 1B-s以及其替代的长转录本PtrWND 1B-l一起表达。PtrWND 1B基因的内含子2结构被鉴定为引起PtrWND 1B选择性剪接的关键序列。抑制PtrWND 1B表达可特异性抑制纤维细胞壁增厚。两种PtrWND 1B亚型在杨树纤维细胞分化过程中对细胞壁增厚的调控中起拮抗作用。PtrWND 1B-s过表达增强纤维细胞壁增厚，而PtrWND 1B-l过表达抑制纤维细胞壁增厚。选择性剪接可以实现对木材形成过程中纤维细胞壁增厚等过程的更具体的调节。
Alternative splicing of a transcription factor generates two isoforms which play antagonistic roles in regulating cell wall thickening during fiber cell differentiation in planta. Alternative splicing is an important mechanism involved in regulating the development of multicellular organisms. Although many genes in plants undergo alternative splicing, little is understood of its significance in regulating plant growth and development. In this study, alternative splicing of black cottonwood (Populus trichocarpa) wood-associated NAC domain transcription factor (PtrWNDs), PtrWND1B, is shown to occur exclusively in secondary xylem fiber cells. PtrWND1B is expressed with a normal short-transcript PtrWND1B-s as well as its alternative long-transcript PtrWND1B-l. The intron 2 structure of the PtrWND1B gene was identified as a critical sequence that causes PtrWND1B alternative splicing. Suppression of PtrWND1B expression specifically inhibited fiber cell wall thickening. The two PtrWND1B isoforms play antagonistic roles in regulating cell wall thickening during fiber cell differentiation in Populus spp. PtrWND1B-s overexpression enhanced fiber cell wall thickening, while overexpression of PtrWND1B-l repressed fiber cell wall thickening. Alternative splicing may enable more specific regulation of processes such as fiber cell wall thickening during wood formation.