Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors
Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors
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DOI：
10.1038/ja.2010.36
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发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Sakuda, Shohei
中科院分区：
文献类型：
--
作者：
Yoshinari, Tomoya;Noda, Yoichi;Sakuda, Shohei
杀稻瘟菌素 A (BcA) 是一种由链霉菌产生的抗生素，可抑制黄曲霉毒素的产生，但对产生黄曲霉毒素的真菌的生长没有强烈的抑制作用。在我们通过二维微分凝胶电泳 (2D-DIGE) 研究 BcA 作用模式的过程中，我们发现酿酒酵母中接触 BcA 后核糖体蛋白的丰度降低。用杀稻瘟菌素 S (BcS) 或放线菌酮处理也观察到这种现象。与 BcS 和放线菌酮类似，BcA 抑制酿酒酵母中半乳糖诱导的表达系统中的蛋白质合成。 BcS（但不是放线菌酮）抑制寄生曲霉中黄曲霉毒素的产生，但不抑制真菌生长，与 BcA 类似。在接触 BcA 或 BcS 后，黄曲霉的 2D-DIGE 实验中观察到黄曲霉毒素生物合成酶的丰度下降。这些结果表明蛋白质合成抑制剂可用于控制黄曲霉毒素的产生。抗生素杂志 (2010) 63, 309-314; doi：10.1038/ja.2010.36； 2010 年 4 月 23 日在线发布
Blasticidin A (BcA), an antibiotic produced by Streptomyces, inhibits aflatoxin production without strong growth inhibition toward aflatoxin-producing fungi. During the course of our study on the mode of action of BcA by two-dimensional differential gel electrophoresis (2D-DIGE), we found a decrease in the abundances of ribosomal proteins in Saccharomyces cerevisiae after exposure to BcA. This phenomenon was also observed by treatment with blasticidin S (BcS) or cycloheximide. BcA inhibited protein synthesis in a galactose-induced expression system in S. cerevisiae similar to BcS and cycloheximide. BcS, but not cycloheximide, inhibited aflatoxin production in Aspergillus parasiticus without inhibition of fungal growth, similar to BcA. A decrease in the abundances of aflatoxin biosynthetic enzymes was observed in 2D-DIGE experiments with Aspergillus flavus after exposure to BcA or BcS. These results suggested that protein synthesis inhibitors are useful to control aflatoxin production. The Journal of Antibiotics (2010) 63, 309-314; doi:10.1038/ja.2010.36; published online 23 April 2010