Dendrites are more spiny on mature hippocampal neurons when synapses are inactivated
Dendrites are more spiny on mature hippocampal neurons when synapses are inactivated
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DOI：
10.1038/13178
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发表时间：
1999-10-01
影响因子：
25
通讯作者：
Harris, KM
中科院分区：
文献类型：
--
作者：
Kirov, SA;Harris, KM
成熟大鼠海马切片中 CA1 锥体神经元的树突暴露于不同水平的突触激活。在一些切片中，突触传递被谷氨酸受体拮抗剂、钠和钙通道阻滞剂和/或名义上不含钙的高镁培养基阻断。在其他切片中，通过低频控制刺激或重复的强直刺激来激活突触。在突触传递受阻的切片中，树突比任何一种激活状态下的树突都多刺。因此，成熟的神经元可以增加其棘的数量，可能补偿失去的突触活动。
Dendrites of CA1 pyramidal neurons in mature rat hippocampal slices were exposed to different levels of synaptic activation. In some slices, synaptic transmission was blocked with glutamate receptor antagonists, sodium and calcium channel blockers and/or a nominally calcium-free medium with high magnesium. In other slices, synapses were activated with low-frequency control stimulation or repeated tetanic stimulation. In slices with blocked synaptic transmission, dendrites were spinier than in either of the activated states. Thus, mature neurons can increase their numbers of spines, possibly compensating for lost synaptic activity.