Timing of neural responses in cortical organotypic slices
Timing of neural responses in cortical organotypic slices
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DOI：
10.1073/pnas.0736909100
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发表时间：
2003-04-15
影响因子：
11.1
通讯作者：
Buonomano, DV
中科院分区：
文献类型：
--
作者：
Buonomano, DV
计时是感觉和运动处理的基本部分。然而，人们对几十到几百毫秒的计时背后的神经机制知之甚少。虽然关于时间处理的可能基础已经提出了许多理论假设，但很少有细胞实验数据，特别是在体外，关于神经机制可能作为计时器的潜在功能。我们使用器官型皮层切片显示，在单次刺激后300 ms内可以观察到可靠的定时动作电位（或兴奋性突触后电位）。延迟反应的潜伏期与与刺激电极的距离没有关系。配对录音和。药理学操作表明，时间延迟反应是活动在功能定义的网络中传播的结果。这些结果表明，皮层网络可能具有处理时间信息的内在能力。
Timing is a fundamental part of sensory and motor processing. However, little is known about the neural mechanisms underlying timing in the range of tens to hundreds of milliseconds. Although many theoretical hypotheses have been put forth on the possible underpinnings of temporal processing, there is little cellular experimental data, particularly in vitro, as to the neural mechanisms that could potentially function as timers. We use organotypic cortical slices to show that reliably timed action potentials (or excitatory postsynaptic potentials) can be observed up to 300 ms after a single stimulus. There was no relationship between the latency of the late responses and distance from the stimulating electrode. Paired recordings and. pharmacological manipulations suggest that the timed late responses are the result of the propagation of activity throughout functionally defined networks. These results show that cortical networks may be intrinsically able to process temporal information.