Colorimetric multiplexed immunoassay using specific aggregation of antigenic peptide-modified luminous nanoparticles.
Colorimetric multiplexed immunoassay using specific aggregation of antigenic peptide-modified luminous nanoparticles.
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DOI：
10.1016/j.aca.2006.04.066
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发表时间：
2006-09
影响因子：
6.2
通讯作者：
T. Ihara;Y. Mori;T. Imamura;M. Mukae;Shojiro Tanaka;A. Jyo
中科院分区：
文献类型：
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作者：
T. Ihara;Y. Mori;T. Imamura;M. Mukae;Shojiro Tanaka;A. Jyo
描述了一种能够一步检测特异性抗体的快速免疫测定法。在各种条件下，通过胱胺和双功能接头分子，将具有阳离子（KKKKC）或阴离子（DDDDC）五聚体尾部的抗原肽固定在40 nm直径（nm）的发光纳米球上。每种多肽锚定在球上的数量分别为5.0× 102和0.8-3.8×103。将FAK和c-Myc抗原肽的混合物固定在具有红色发射的微球上，而将c-Myc和α-catenin抗原肽的混合物固定在具有绿色发射的微球上。通过在适当的条件下将抗体加入到这些球的混合分散溶液中，可以容易地实现多重免疫测定。抗FAK和抗α-连环蛋白抗体分别形成具有红色和绿色发射的聚集体。另一方面，抗c-Myc抗体形成发射黄光的聚集体。该系统使我们能够区分一个容器中的三种抗体，从聚集体颜色的明确差异。
A rapid immunoassay capable of detecting specific antibodies in one-step procedure is described. Antigenic peptides with cationic (KKKKC) or anionic (DDDDC) pentamer tail were immobilized on luminous nanospheres of 40nm diameter (Ø) through cystamine and bifunctional linker molecules under various conditions. The numbers of each peptide anchored to a sphere were 5.0×102and 0.8–3.8×103, respectively. A mixture of the antigenic peptides of FAK and c-Myc was immobilized to the spheres with red emission, while that of c-Myc and α-catenin was likewise to green spheres. Multiplexed immunoassay was easily achieved by adding the antibodies to a mixed dispersed solution of these spheres under appropriate conditions. Anti-FAK and anti-α-catenin antibodies formed aggregates with red and green emissions, respectively. On the other hand, the anti-c-Myc antibody formed aggregates emitting a yellow light. This system enabled us to differentiate three antibodies in one vessel from the definite differences in aggregate color.