Compared responses of poplar cuttings and in vitro raised shoots to short-term chilling treatments
Compared responses of poplar cuttings and in vitro raised shoots to short-term chilling treatments
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DOI：
10.1007/s002990000229
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发表时间：
2000-10-01
影响因子：
6.2
通讯作者：
Jouve, L
中科院分区：
文献类型：
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作者：
Hausman, JF;Evers, D;Jouve, L
在木本植物中，低温胁迫发生在早春生长期间，并可能产生重要的经济后果。本研究的目的是比较两种不同的实验系统，即3个月生的嫩枝和试管苗的可靠性，以研究杨树无性系的低温效应。-1)。在10℃处理2周期间，记录了不同的参数：木质素含量、蔗糖浓度、蛋白质和鲜重的变化。结果表明，插条和试管苗中均有高分子量多肽(110 kDa和116 kDa)的积累。对于所研究的一些参数，如某些多肽的出现和鲜重增加的减少，体外系统和体外系统都是适用的，并产生了相似的结果。相反，对于其他一些观察，即蔗糖浓度和木质素含量，系统得出了不同的结论。插条经低温处理后，蔗糖和木质素含量增加，而木质素含量变化不大，只有蔗糖的一个小的暂时性峰值。这些参数似乎不适合在如此短期的研究中研究杨树在体外系统中的反应。
In woody plants, chilling stress occurs during the early spring growth and can have important economic consequences. The aim of this study was to compare the reliability of two different experimental systems, 3-month-old softwood cuttings and in vitro-grown shoots, to study chilling effects in a poplar clone (Populus tremula x P. tremuloides cv. Muhs1). Different parameters were recorded: lignin content, sucrose concentration and protein and fresh weight variation, during a 2-week treatment at 10 degreesC. Two families of polypeptides of high molecular weight (110 and 116 kDa) were shown to accumulate in response to chilling in both cuttings and microshoots. For some of the parameters studied, i.e. appearance of some groups of polypeptides and reduction of fresh weight gain, both in vitro and ex vitro systems were suitable and produced similar results. In contrast, for some other observations, i.e. on sucrose concentration and lignin content, the systems led to different conclusions. While sucrose and lignin contents were shown to increase in cuttings submitted to chilling, no variation in lignin and only a small temporary peak of sucrose could be observed in microshoots kept under chilling. These parameters seem not to be suitable for studying the response of poplar in the in vitro system in such a shortterm study.