EPIDERMAL GROWTH-FACTOR AND BOVINE GROWTH-HORMONE STIMULATE DIFFERENTIATION AND MYELINATION OF BRAIN-CELL AGGREGATES IN CULTURE
EPIDERMAL GROWTH-FACTOR AND BOVINE GROWTH-HORMONE STIMULATE DIFFERENTIATION AND MYELINATION OF BRAIN-CELL AGGREGATES IN CULTURE
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DOI：
10.1016/0165-3806(85)90214-7
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
GUENTERTLAUBER, B
中科院分区：
文献类型：
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作者：
ALMAZAN, G;HONEGGER, P;GUENTERTLAUBER, B
牛生长激素（bGH）和表皮生长因子（EGF）增加了在无血清化学成分确定的培养基中培养的脑细胞聚集体中鸟氨酸脱羧酶（ODC）的活性。ODC被认为是细胞生长和分化的标志物。通过测定2种髓鞘标记物2 ''，3 ''-环核苷酸-3 ''-磷酸二酯酶（CNP）和髓鞘碱性蛋白（MBP）来研究bGH和EGF对髓鞘形成的影响。EGF治疗在第2天和第5天引起的剂量依赖性增加的两个髓鞘标记物在培养第12天。在培养第19天仍然可以观察到这种增加，表明EGF的作用延长。在第19天，培养基中bGH的持续存在产生了大量的MBP积累。这种效应是剂量依赖性的，需要三碘甲状腺原氨酸（T3）的存在。bGH对CNP活性的影响不需要存在T3。这是第一份报告显示bGH对体外CNS髓鞘形成和EGF对MBP积累和ODC活性的直接影响。
Bovine growth hormone (bGH) and epidermal growth factor (EGF) increased the activity of ornithine decarboxylase (ODC) in brain cell aggregates cultured in a serum-free chemically defined medium. ODC is considered as a marker of cell growth and differentiation. The effect of bGH and EGF on myelination was investigated by measuring 2 myelin markers, 2'',3''-cyclic nucleotide-3''-phosphodiesterase (CNP) and myelin basic protein (MBP). EGF treatment at days 2 and 5 caused a dose-dependent increase of both myelin markers at culture day 12. This increase could still be observed at culture day 19, indicating a prolonged action of EGF. The continual presence of bGH in the culture medium produced a large accumulation of MBP at day 19. This effect was dose-dependent and required the presence of triiodothyronine (T3). The effect of bGH on CNP activity did not require the presence of T3. This is the first report showing a direct effect of bGH on CNS myelination in vitro and of EGF on both MBP accumulation and ODC activity.