Temporal regulation of late expression of Bar homeobox genes during Drosophila leg development by Spineless, a homolog of the mammalian dioxin receptor
Temporal regulation of late expression of Bar homeobox genes during Drosophila leg development by Spineless, a homolog of the mammalian dioxin receptor
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DOI：
10.1016/j.ydbio.2006.03.015
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发表时间：
2006-06-15
影响因子：
2.7
通讯作者：
Kojima, Tetsuya
中科院分区：
文献类型：
--
作者：
Kozu, Shintaro;Tajiri, Reiko;Kojima, Tetsuya
编码转录因子的基因的时空调控对于多细胞生物的正常发育是必不可少的。在果蝇腿发育中，最远端的跗骨（ta 5）是由一对Bar同源盒基因在三龄后期的强表达所指定的。该表达在由Bar激活的ta 5增强子的控制下调节。没有激活的ta 5增强子，但是，发生在早期的第三龄时，相当大的酒吧生产。ta 5增强子由驱动Bar表达所需的基础增强子和负调控基序组成，所述负调控基序分别用作哺乳动物二恶英受体和芳烃核转位器的同源物Spineless和Tango的异二聚体的结合位点。无刺和探戈是抑制三龄早期基础增强子激活所必需的。无刺蛋白在三龄早期的Bar表达域中瞬时表达。因此，ta 5 Bar表达可以通过短暂抑制基础增强子的过早激活来进行时间调节，所述短暂抑制是通过无刺/Tango异源二聚体与三龄早期的负调节基序的特异性结合以及随后由于在后期无刺表达的消失而从抑制中释放来进行的。(c)2006年爱思唯尔公司All rights reserved.
The spatial and temporal regulation of genes encoding transcription factors is essential for the proper development of multicellular organisms. In Drosophila leg development, the distal-most tarsus (ta5) is specified by the strong expression of a pair of Bar homeobox genes in late third instar. This expression is regulated under the control of the ta5 enhancer activated by Bar. No activation of the ta5 enhancer, however, occurs in early third instar when considerable Bar is produced. The ta5 enhancer was comprised of a basal enhancer required for driving Bar expression and a negative regulatory motif serving as a binding site for the heterodimer of Spineless and Tango, homologs of the mammalian dioxin receptor and aryl hydrocarbon nuclear translocator, respectively. The spineless and tango were essential for suppressing the basal enhancer activation in early third instar. The spineless was transiently expressed in early third instar in the Bar expression domain. ta5 Bar expression may thus be temporally regulated through transient inhibition of premature activation of the basal enhancer via specific binding of the Spineless/Tango heterodimer to the negative regulatory motif in early third instar and subsequent release from the inhibition due to the disappearance of spineless expression at later stages. (c) 2006 Elsevier Inc. All rights reserved.