Effects of glucagon on glycogenolysis and gluconeogenesis ore region-specific in periportal and perivenous hepatocytes
Effects of glucagon on glycogenolysis and gluconeogenesis ore region-specific in periportal and perivenous hepatocytes
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DOI：
10.1016/s0022-2143(98)90134-2
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发表时间：
1998-12-01
期刊：
影响因子：
--
通讯作者：
Sasaki, H
中科院分区：
文献类型：
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作者：
Ikezawa, Y;Yamatani, K;Sasaki, H
主要通过组织化学和免疫组织化学研究已经确定，肝细胞在功能上是异质的，门静脉周围肝细胞（PPH）主要是生血管的，静脉周围肝细胞（PVH）是糖酵解的。因此，我们研究了胰高血糖素对通过毛地黄皂苷-胶原酶方法制备的分离的PPH和PVH中糖原分解和糖原异生的区域特异性功能效应。采用胰岛素依赖型糖尿病模型BB大鼠，研究糖尿病状态下血管新生的区域特异性异质性。虽然正常饮食大鼠中PVH和PPH之间的糖原含量没有差异，但PVH的基础葡萄糖释放量是PPH的1.37倍（P
It has been established, mainly by histochemical and immunohistochemical studies, that liver cells are functionally heterogeneous, with periportal hepatocytes (PPHs) being predominantly gluconeogenic and perivenous hepatocytes (PVHs) being glycolytic. We therefore investigated the region-specific functional effects of glucagon on glycogenolysis and gluconeogenesis in isolated PPHs and PVHs prepared by the digitonin-collagenase method. BB rats, a model of insulin-dependent diabetes, were used to study the region-specific heterogeneity of gluconeogenesis in the diabetic state. Although glycogen content was not different between PVHs and PPHs in rats fed the normal diet, basal glucose release was 1.37 times greater in PVHs than in PPHs (P