Salmonella serovar identification using PCR-based detection of gene presence and absence
Salmonella serovar identification using PCR-based detection of gene presence and absence
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DOI：
10.1128/jcm.02147-07
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发表时间：
2008-08-01
影响因子：
9.4
通讯作者：
McClelland, Michael
中科院分区：
文献类型：
--
作者：
Arrach, Nabil;Porwollik, Steffen;McClelland, Michael
已知的沙门氏菌有2500多个血清型，其中一些可以进一步细分为基因含量差异很大的菌株组。我们把这些菌株称为“基因变种”。收集了291株沙门氏菌的比较基因组杂交数据，其中包括32个血清型(52个基因组)中的250个肠出血性沙门氏菌亚种I菌株，从中筛选出具有潜在分类价值的384个基因。用146个基因的子集进行实时荧光定量聚合酶链式反应，成功地鉴定了24株肠杆菌的12个血清型(16个基因组)。利用基因的另一个子集对11株肠球菌的12个多重聚合酶链式反应中的8个血清型(9个基因组)进行了鉴定。这些基因板几乎都通过两个或两个以上的信息性标记来区分所有测试的肠出血性葡萄球菌I亚种及其已知的基因组。因此，基于这些预测基因的分型方法通常会在出现错误、意外的多态或潜在的新基因变种时提醒用户。
There are more than 2,500 known Salmonella serovars, and some of these can be further subclassified into groups of strains that differ profoundly in their gene content. We refer to these groups of strains as "genovars." A compilation of comparative genomic hybridization data on 291 Salmonella isolates, including 250 S. enterica subspecies I strains from 32 serovars ( 52 genovars), was used to select a panel of 384 genes whose presence and absence among serovars and genovars was of potential taxonomic value. A subset of 146 genes was used for real-time PCR to successfully identify 12 serovars ( 16 genovars) in 24 S. enterica strains. A further subset of 64 genes was used to identify 8 serovars ( 9 genovars) in 12 multiplex PCR mixes on 11 S. enterica strains. These gene panels distinguish all tested S. enterica subspecies I serovars and their known genovars, almost all by two or more informative markers. Thus, a typing methodology based on these predictive genes would generally alert users if there is an error, an unexpected polymorphism, or a potential new genovar.