The Brevibacterium flavum sigma factor SigB has a role in the environmental stress response
The Brevibacterium flavum sigma factor SigB has a role in the environmental stress response
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DOI：
10.1111/j.1574-6968.2002.tb11418.x
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发表时间：
2002-10-29
影响因子：
2.1
通讯作者：
Kormanec, J
中科院分区：
文献类型：
--
作者：
Halgasova, N;Bukovska, G;Kormanec, J
我们之前已经克隆了一个编码SigB的基因，SigB是黄色短杆菌中的一个主要的类Sigma因子，它是在几种胁迫条件下诱导的。为了阐明Sigma因子在体内的功能，SIGB基因被同源重组打乱，用卡那霉素抗性标记基因取代了黄色芽孢杆菌染色体上的内部必要编码区。该突变极大地降低了黄杆菌的营养生长速度。对SIGB突变对细胞生长和活力在应激条件下的影响的研究表明，SIGB突变增加了对酸、盐、酒精、热和冷应激的敏感性。质粒生的野生型SIGB基因补充了突变。根据这一结果，我们认为SigB在营养生长和对各种环境胁迫的响应中具有一定的作用。(C)2002年欧洲微生物学会联合会。爱思唯尔科学公司出版。版权所有。
We have previously cloned a gene encoding a SigB, a principal-like sigma factor in Brevibacterium flavum, which was induced by several stress conditions. To clarify the in vivo function of this sigma factor, the sigB gene was disrupted by a homologous recombination, replacing the internal essential coding region in B. flavum chromosome by a kanamycin resistance marker gene. This mutation dramatically decreased vegetative growth rates of B. flavum. Studies of the effect of the sigB mutation on growth and viability of the cells under conditions of stress showed that the sigB mutant had increased susceptibility to acid, salt, alcohol, heat and cold stress. The plasmid-born wild-type sigB gene complemented the mutation. Based on the results, we propose that SigB has a role in vegetative growth and in response to various environmental stresses. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.