Effect of defensin peptides on eukaryotic cells: primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines
Effect of defensin peptides on eukaryotic cells: primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines
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DOI：
10.1016/j.jdermsci.2004.07.001
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发表时间：
2004-11-01
影响因子：
4.6
通讯作者：
Kaku, T
中科院分区：
文献类型：
--
作者：
Nishimura, M;Abiko, Y;Kaku, T
背景资料：虽然已知为防御素的抗微生物肽的有用性已被建议对抗口腔和皮肤感染，但在考虑临床应用之前，应了解防御素对宿主可能的不利影响。在本研究中，我们研究了α-防御素（HNP-1）和β-防御素（hBD-1、-2、-3）影响细胞，包括原代上皮细胞、成纤维细胞和鳞状细胞癌细胞系SCC-9和KB。方法：通过直接细胞计数和XTT试验评估细胞增殖。结果：我们发现，α-防御素在低浓度下促进上皮细胞增殖，但在高浓度下具有细胞毒性作用。相比之下，β-防御素在任何浓度下对这些细胞的影响都很小，这表明β-防御素可能对宿主没有不利影响。结论：因此，就宿主反应而言，β-防御素可能比α-防御素更适合于临床应用的抗菌剂。(C)2004年日本皮肤病研究学会。由Elsevier爱尔兰有限公司出版。保留所有权利。
Background: Although the usefulness of the antimicrobial peptides known as defensins has been suggested against oral and skin infections, possible adverse effects of the defensins on the host should be understood before clinical applications can be contemplated.Objective: In the present study, we investigated how alpha-defensin (HNP-1) and beta-defensins (hBD-1, -2, -3) affect cells including primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines, SCC-9 and KB.Method: Cell proliferation was assessed by the direct cell counting and XTT assay.Results: We found that a-defensin promotes proliferation of the epithelial cells at low concentration but has a cytotoxic effect at high concentration. In contrast, beta-defensins have little effect on these cells at any concentration, suggesting that beta-defensins may have no adverse effects on the host.Conclusion: Therefore, in terms of host response beta-defensins may be more suitable antimicrobial agents for clinical applications than alpha-defensins. (C) 2004 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.