Non‐enzymatic glycosylation of keratin from the stratum corneum of the diabetic foot
Non‐enzymatic glycosylation of keratin from the stratum corneum of the diabetic foot
复制标题
糖尿病足角质层角蛋白的非酶糖基化
DOI：
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发表时间：
1985
影响因子：
10.3
通讯作者：
L. Lequesne
中科院分区：
文献类型：
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作者：
L. Delbridge;C. S. Ellis;K. Robertson;L. Lequesne
通过硫代巴比妥酸技术在 30 名糖尿病受试者和 30 名对照受试者中测量了脚底角质层角蛋白的非酶糖基化。糖尿病受试者的糖基化水平显着增加（P < 0.001）。在去除角蛋白的同时，对另外 18 名受试者的 HbA1 水平进行了测量，在该组中，该蛋白质的非酶糖基化与糖尿病控制之间存在显着关联（P < 0.01）。在存在游离葡萄糖的情况下体外孵育角蛋白会产生增加的非酶糖基化（P < 0.01），并且在存在浓度增加的阿司匹林的情况下孵育会阻断这种效应（P < 0.01）。对另一组 20 名患有神经性溃疡的糖尿病患者进行的角蛋白非酶糖基化测量显示，与一组没有溃疡的糖尿病患者相比，角蛋白的非酶糖基化水平显着升高（P < 0.05）。由于角蛋白是脚底角质层的主要结构蛋白，因此这种与非酶糖基化相关的蛋白质的变化可能会导致糖尿病患者足部皮肤的异常。
Non‐enzymatic glycosylation of keratin from the stratum corneum of the sole of the foot was measured by the thiobarbituric acid technique in thirty diabetic and thirty control subjects. A significant increase in the level of glycosylation was demonstrated in the diabetic subjects (P < 0.001). HbA1 levels were measured in a further eighteen subjects at the same time as keratin was removed, and in this group a significant association between non‐enzymatic glycosylation of that protein and diabetic control was demonstrated (P < 0.01). In vitro incubation of keratin in the presence of free glucose produced increased non‐enzymatic glycosylation (P < 0.01) and this effect was blocked by incubation in the presence of increasing concentrations of aspirin (P < 0.01). Measurement of non‐enzymatic glycosylation of keratin in a further group of twenty diabetics with neuropathic ulceration showed a significant increase in levels when compared with a group of diabetics without ulcers (P < 0.05). As keratin is the principle structural protein of the stratum corneum of the sole of the foot, it is possible that changes in this protein associated with non‐enzymatic glycosylation may contribute to abnormalities seen in the skin of the feet of diabetics.