Two homologous low-temperature-inducible genes from Arabidopsis encode highly hydrophobic proteins
Two homologous low-temperature-inducible genes from Arabidopsis encode highly hydrophobic proteins
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DOI：
10.1104/pp.115.2.569
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发表时间：
1997-10-01
期刊：
影响因子：
7.4
通讯作者：
MartinezZapater, JM
中科院分区：
文献类型：
--
作者：
Capel, J;Jarillo, JA;MartinezZapater, JM
我们已经表征了两个相关的cDNA（RCI2A和RCI2B）对应的基因从拟南芥，其表达是瞬时诱导的低，非冻结温度。RCI2A和RCI2B编码小的（54个氨基酸），高度疏水的蛋白质，具有两个潜在的跨膜结构域。它们与大麦（Hordeum volgare L.）和冰草（Lophophyrom elongatum），其受不同胁迫条件调节。它们的高水平的序列同源性（78%）和它们在单个限制性片段中的基因组位置表明这两个基因起源于串联重复的结果。然而，它们的调节序列已经足够多样化，从而赋予它们不同的表达模式。与大多数冷诱导植物基因一样，RCI 2A和RCI 2B的表达也受到脱落酸（阿坝）和脱水的促进，但不是对胁迫条件的一般反应，因为它不是由盐胁迫或缺氧诱导的。此外，低温能够诱导RCI2A和RCI2B在ABA缺乏和不敏感的遗传背景中表达，表明ABA依赖和非依赖途径调节这两个基因的低温响应性。
We have characterized two related cDNAs (RCI2A and RCI2B) corresponding to genes from Arabidopsis thaliana, the expression of which is transiently induced by low, nonfreezing temperatures. RCI2A and RCI2B encode small (54 amino acids), highly hydrophobic proteins that bear two potential transmembrane domains. They show similarity to proteins encoded by genes from barley (Hordeum volgare L.) and wheatgrass (Lophophyrom elongatum) that are regulated by different stress conditions. Their high level of sequence homology (78%) and their genomic location in a single restriction fragment suggest that both genes originated as a result of a tandem duplication. However, their regulatory sequences have diverged enough to confer on them different expression patterns. Like most of the cold-inducible plant genes characterized, the expression of RCI2A and RCI2B is also promoted by abscisic acid (ABA) and dehydration but is not a general response to stress conditions, since it is not induced by salt stress or by anaerobiosis. Furthermore, low temperatures are able to induce RCI2A and RCI2B expression in ABA-deficient and -insensitive genetic backgrounds, indicating that both ABA-dependent and -independent pathways regulate the low-temperature responsiveness of these two genes.