HYALURONIC-ACID IN HUMAN ARTICULAR-CARTILAGE - AGE-RELATED-CHANGES IN CONTENT AND SIZE
HYALURONIC-ACID IN HUMAN ARTICULAR-CARTILAGE - AGE-RELATED-CHANGES IN CONTENT AND SIZE
复制标题
DOI：
10.1042/bj2500435
复制
发表时间：
1988-03-01
影响因子：
4.1
通讯作者：
MUIR, H
中科院分区：
文献类型：
--
作者：
HOLMES, MWA;BAYLISS, MT;MUIR, H
使用灵敏的放射性吸附测定法测定人关节软骨的木瓜蛋白酶底物中透明质酸的总组织含量和分子量[Laurent和Tengblad（1980）Anal. 109，386-394]。1)透明质酸含量从0.5 μ g/mg湿重增加到0.5 μ g/mg湿重。至2.5 μ g/mg湿重。年龄在2.5岁到86岁之间。2)透明质酸链尺寸从Mr2.0 ×106至3.0 ×105岁以上的人。3)在未成熟和成熟的软骨中，新合成的透明质酸的大小没有与年龄相关的变化，透明质酸具有非常高的分子量。结果是一致的年龄相关的蛋白聚糖聚集体的大小减少，并建议透明质酸链的修改可能发生在细胞外基质。
Total tissue content and molecular mass of hyaluronic acid was determined in papain digests of human articular cartilage using a sensitive radiosorbent assay [Laurent and Tengblad (1980) Anal. Biochem. 109, 386-394]. 1) Hyaluronic acid content increased from 0.5 .mu.g/mg wet wt. to 2.5 .mu.g/mg wet wt. between the ages of 2.5 years and 86 years. 2) Hyaluronic acid chain size decreased from Mr 2.0 .times. 106 to 3.0 .times. 105 over the same age range. 3) There was no age-related change in the size of newly-synthesized hyaluronic acid, which was of very high molecular mass, in both immature and mature cartilage. The results are consistent with an age-related decrease in proteoglycan aggregate size and suggest that modification of the hyaluronic acid chain may take place in the extracellular matrix.