PURIFICATION AND CHARACTERIZATION OF DNA-DEPENDENT RNA-POLYMERASE FROM STAPHYLOCOCCUS-AUREUS
PURIFICATION AND CHARACTERIZATION OF DNA-DEPENDENT RNA-POLYMERASE FROM STAPHYLOCOCCUS-AUREUS
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DOI：
10.1006/bbrc.1995.1382
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发表时间：
1995-03-17
影响因子：
3.1
通讯作者：
MISRA, TK
中科院分区：
文献类型：
--
作者：
DEORA, R;MISRA, TK
从指数生长的金黄色葡萄球菌细胞中纯化DNA依赖性RNA聚合酶。对最纯的制备物进行SDS-聚丙烯酰胺凝胶分析，结果表明它由表观分子量分别为151、147、42和55 kDa的β、β ′、α和σ组成。σ亚基与抗枯草芽孢杆菌σ的多克隆抗体交叉反应（43）。交叉反应肽与B共同迁移。subtilis sigma（43）。亚单位这些结果的影响进行了讨论。使用纯化的酶制剂获得启动子特异性体外径流转录物。定义了聚合反应的具体条件。(C)出版社：Academic Press
DNA dependent RNA polymerase from exponentially growing Staphylococcus aureus cells was purified. An SDS-polyacrylamide gel analysis of the most purified preparation revealed that it consists of beta, beta', alpha, and sigma with apparent molecular masses of 151, 147, 42, and 55 kDa, respectively. The sigma subunit cross reacted with a polyclonal antibody against Bacillus subtilis sigma(43). The cross reacting peptide co-migrated with the B. subtilis sigma(43). subunit. The implications of these results are discussed. Promoter specific in vitro run-off transcripts were obtained using the purified enzyme preparation. Specific conditions for the polymerization reaction are defined. (C) 1995 Academic Press, Inc.