Enhanced osteogenic differentiation of mesenchymal stem cells on poly(L-lactide) nanofibrous scaffolds containing carbon nanomaterials
Enhanced osteogenic differentiation of mesenchymal stem cells on poly(L-lactide) nanofibrous scaffolds containing carbon nanomaterials
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DOI：
10.1002/jbm.a.35283
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发表时间：
2015-04-01
影响因子：
4.9
通讯作者：
Cai, Qing
中科院分区：
文献类型：
--
作者：
Duan, Shun;Yang, Xiaoping;Cai, Qing
碳纳米材料，如碳纳米管（CNT）和石墨烯，由于其骨诱导特性而在骨再生中突出显示。它们与纳米纤维聚合物支架的结合，模仿了骨的天然细胞外基质的形态，引起了骨组织工程的浓厚兴趣。为此，通过热诱导相分离将CNM掺入纳米纤维状聚（l-乳酸）支架中。通过骨髓间充质干细胞（BMSCs）体外共培养和体内植入两种方法对含CNM的复合纳米纤维支架进行生物学评价。纳米纤维结构本身表现出显着的细胞粘附，增殖和成骨分化的BMSCs的增强，并与CNM的掺入，复合纳米纤维支架进一步促进BMSCs的成骨分化显着。在两种纳米材料中，石墨烯对BMSCs成骨分化的促进作用强于CNT。体内实验结果表明，复合纳米纤维支架具有良好的生物相容性和较强的诱导成骨能力。CNMs能显著促进成骨相关蛋白的表达和I型胶原的形成。类似地，含石墨烯的复合纳米纤维支架在体内诱导成骨方面表现出最强的效果。这些发现表明，含CNM的复合纳米纤维支架在促进成骨方面明显比纯聚合物支架更有效。(c)2014 Wiley Periodicals，Inc. J Biomed Mater Res Part A：103A：1424 - 1435，2015.
Carbon nanomaterials (CNMs), such as carbon nanotube (CNT) and graphene, are highlighted in bone regeneration because of their osteoinductive properties. Their combinations with nanofibrous polymeric scaffolds, which mimic the morphology of natural extracellular matrix of bone, arouse keen interest in bone tissue engineering. To this end, CNM were incorporated into nanofibrous poly(l-lactic acid) scaffolds by thermal-induced phase separation. The CNM-containing composite nanofibrous scaffolds were biologically evaluated by both in vitro co-culture of bone mesenchymal stem cells (BMSCs) and in vivo implantation. The nanofibrous structure itself demonstrated significant enhancement in cell adhesion, proliferation and oseogenic differentiation of BMSCs, and with the incorporation of CNM, the composite nanofibrous scaffolds further promoted osteogenic differentiation of BMSCs significantly. Between the two CNMs, graphene showed stronger effect in promoting osteogenic differentiation of BMSCs than CNT. The results of in vivo experiments revealed that the composite nanofibrous scaffolds had both good biocompatibility and strong ability in inducing osteogenesis. CNMs could remarkably enhance the expression of osteogenesis-related proteins as well as the formation of type I collagen. Similarly, the graphene-containing composite nanofibrous scaffolds demonstrated the strongest effect on inducing osteogenesis in vivo. These findings demonstrated that CNM-containing composite nanofibrous scaffolds were obviously more efficient in promoting osteogenesis than pure polymeric scaffolds. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1424-1435, 2015.