Cancer-testis antigen expression is shared between epithelial ovarian cancer tumors
Cancer-testis antigen expression is shared between epithelial ovarian cancer tumors
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DOI：
10.1016/j.ygyno.2017.03.512
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发表时间：
2017-06-01
影响因子：
4.7
通讯作者：
Podack, Eckhard R.
中科院分区：
文献类型：
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作者：
Garcia-Soto, Arlene E.;Schreiber, Taylor;Podack, Eckhard R.
目标。肿瘤睾丸（CT）抗原被认为是癌症免疫治疗的潜在靶点。我们的目的是评估一组CT抗原在上皮性卵巢癌（EOC）肿瘤标本中的表达，并确定肿瘤之间是否发生抗原共享。方法从卵巢癌肿瘤标本、卵巢癌细胞系和卵巢良性组织标本中分离RNA。采用Real - time-PCR法检测20个CT抗原的表达水平。对62例卵巢癌标本、8个卵巢癌细胞系和3个良性卵巢组织进行CT抗原表达检测。多数标本为：高等级（62%）、浆液性（68%）和晚期（74%）。58例（95%）的EOC肿瘤至少表达一种被评估的CT抗原。CT抗原平均表达4.5个（0 ~ 17个）。最常表达的CT抗原为MAGE A4（65%）。抗原共享分析结果如下：9例肿瘤仅共享一种抗原，占评估标本的62%；37例肿瘤共享4种及以上抗原，占评估标本的82%。5例肿瘤表达10种以上的CT抗原，90%的肿瘤组有相同的抗原表达。CT抗原在95%的EOC肿瘤标本中表达。然而，没有一种抗原在所有样本中普遍表达。随着抗原表达总数的增加，肿瘤间抗原共享程度增加。这些数据提示了卵巢癌免疫治疗的多表位方法。(C) 2017爱思唯尔公司版权所有。
Objectives. Cancer-testis (CT) antigens have been proposed as potential targets for cancer immunotherapy. Our objective was to evaluate the expression of a panel of CT antigens in epithelial ovarian cancer (EOC) tumor specimens, and to determine if antigen sharing occurs between tumors.Methods, RNA was isolated from EOC tumor specimens, EOC cell lines and benign ovarian tissue specimens. Real time-PCR analysis was performed to determine the expression level of 20 CT antigens.Results. A total of 62 EOC specimens, 8 ovarian cancer cell lines and 3 benign ovarian tissues were evaluated for CT antigen expression. The majority of the specimens were: high grade (62%), serous (68%) and advanced stage (74%). 58 (95%) of the EOC tumors analyzed expressed at least one of the CT antigens evaluated. The mean number of CT antigen expressed was 4.5 (0-17). The most frequently expressed CT antigen was MAGE A4 (65%). Antigen sharing analysis showed the following: 9 tumors shared only one antigen with 62% of the evaluated specimens, while 37 tumors shared 4 or more antigens with 82%. 5 tumors expressed over 10 CT antigens, which were shared with 90% of the tumor panel.dConclusion. CT antigens are expressed in 95% of EOC tumor specimens. However, not a single antigen was universally expressed across all samples. The degree of antigen sharing between tumors increased with the total number of antigens expressed. These data suggest a multi-epitope approach for development of immunotherapy for ovarian cancer treatment. (C) 2017 Elsevier Inc. All rights reserved.