Genotoxicity of Zinc Oxide Nanoparticles in Plants Demonstrated Using Transgenic Arabidopsis thaliana
Genotoxicity of Zinc Oxide Nanoparticles in Plants Demonstrated Using Transgenic Arabidopsis thaliana
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DOI：
10.1007/s00128-018-2420-7
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发表时间：
2018-08
影响因子：
2.7
通讯作者：
Aifeng Yang;Jingjing Wu;Chenguang Deng;Ting Wang;P. Bian
中科院分区：
文献类型：
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作者：
Aifeng Yang;Jingjing Wu;Chenguang Deng;Ting Wang;P. Bian
高等植物作为地球生物圈的重要成员，不可避免地会暴露在释放到环境中的纳米粒子（NP）中。因此，确定NP诱导的植物毒性在生态学上是重要的。目前，NP对植物的遗传毒性研究还很有限。本研究首次采用转同源重组（HR）和转录基因沉默（TGS）报告基因的拟南芥（Arabidopsis thalianalines）评价氧化锌纳米粒（ZnO-NP）的遗传毒性。结果表明，根暴露于ZnO-NP导致增加HR和减轻TGS在气生组织中，指示ZnO-NP在植物中的遗传毒性。ZnO-NP处理后根中Zn含量的增加以及等量Zn离子处理后根中HR和TGS的缓解作用表明，ZnO-NP的遗传毒性可能主要是由Zn离子在地上组织中的转运引起的。
As important members of earth biosphere, higher plants are inevitably exposed to nanoparticles (NP) released into the environment. Therefore, determining NP-induced phytotoxicity is ecologically important. Currently, researches into genotoxic effects of NP on plants are limited. In this study,Arabidopsis thalianalines transgenic for homologous recombination (HR) and transcriptional gene silencing (TGS) reporter genes were for the first time adopted to assess the genotoxicity of Zinc oxide NP (ZnO–NP). Results showed that the root exposure to ZnO–NP led to increased HR and alleviation of TGS in the aerial tissues, indicative of the genotoxicity of ZnO–NP in plants. The increased Zn content after root exposure to ZnO–NP and the similar induction of HR and TGS alleviation after root exposure to equivalent Zn ions suggested that the genotoxicity of ZnO–NP might be mainly induced by Zn ions in aerial tissues that were transported from decomposed ZnO–NP in either medium or plant roots.