Distribution of glycinergic neuronal somata in the rat spinal cord
Distribution of glycinergic neuronal somata in the rat spinal cord
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DOI：
10.1016/j.brainres.2007.01.078
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发表时间：
2007-04-20
期刊：
影响因子：
2.9
通讯作者：
Holstege, Jan C.
中科院分区：
文献类型：
--
作者：
Hossaini, Mehdi;French, Pim J.;Holstege, Jan C.
甘氨酸转运体2（GlyT 2）mRNA仅在甘氨酸能神经元中表达，并且目前被认为是甘氨酸能神经元胞体的可靠标志物。在这项研究中，我们进行了非放射性原位杂交定位GlyT 2 mRNA在固定的自由浮动切片的颈（C2和C6），胸（TS），腰（L2和L5）和骶（Si）的大鼠脊髓。结果表明，在所有节段中，大多数GlyT 2 mRNA标记的（甘氨酸能）神经元胞体存在于深背角和中间区（层III-VIII），其中约50%（范围43.7-70.9%）存在于层VII和VIII。相反，背角浅层、运动神经元群和中央管周围区域仅含有少量甘氨酸能神经元胞体。这些区域的密度（每毫米（2）甘氨酸能神经元胞体的数量）也较低，而最高密度出现在第V层至第VIII层。脊髓外侧核和颈外侧核也含有数量有限的甘氨酸能神经元。我们的研究结果表明，甘氨酸能神经元胞体的分布模式是相似的，在所有的检查节段。在一些节段之间的相对层流分布中发现的少数差异很可能是由于技术原因。因此，我们得出结论，所观察到的分布模式的甘氨酸能神经元胞体是整个脊髓。我们的研究结果进一步表明，非放射性原位杂交技术，用于确定GlyT 2 mRNA在固定的自由浮动部分是一个非常有效的工具，用于确定脊髓中的甘氨酸能神经元。(c)2007 Elsevier B. V.保留所有权利。
Glycine transporter 2 (GlyT2) mRNA is exclusively expressed in glycinergic neurons, and is presently considered a reliable marker for glycinergic neuronal somata. in this study, we have performed non-radioactive in situ hybridization to localize GlyT2 mRNA in fixed free-floating sections of cervical (C2 and C6), thoracic (TS), lumbar (L2 and L5) and sacral (Si) segments of the rat spinal cord. The results showed that in all segments the majority of the GlyT2 mRNA labeled (glycinergic) neuronal somata was present in the deep dorsal horn and the intermediate zone (laminae III-VIII), with around 50% (range 43.7-70.9%) in laminae VII&VIII. in contrast, the superficial dorsal horn, the motoneuronal cell groups and the area around the central canal contained only few glycinergic neuronal somata. The density (number of glycinergic neuronal somata per mm(2)) was also low in these areas, while the highest densities were found in laminae V to VIII. The lateral spinal nucleus and the lateral cervical nucleus also contained a limited number of glycinergic neurons. Our findings showed that the distribution pattern of the glycinergic neuronal somata is similar in all the examined segments. The few differences that were found in the relative laminar distribution between some of the segments, are most likely due to technical reasons. We therefore conclude that the observed distribution pattern of glycinergic neuronal somata is present throughout the spinal cord. Our findings further showed that the non-radioactive in situ hybridization technique for identifying GlyT2 mRNA in fixed free-floating sections is a highly efficient tool for identifying glycinergic neurons in the spinal cord. (c) 2007 Elsevier B.V. All rights reserved.