Identification of interspecific hybrids by amplified fragment length polymorphism: the case of sturgeon

Identification of interspecific hybrids by amplified fragment length polymorphism: the case of sturgeon
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DOI:
10.1046/j.0962-1083.2001.01368.x
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发表时间:
2001-09-01
期刊:
影响因子:
4.9
通讯作者:
Zane, L
Zane, L
中科院分区:
生物学1区
文献类型:
--
作者:
Congiu, L;Dupanloup, I;Zane, L

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种间杂种的鉴定是保护生物学和贸易控制的一个重要问题。在意大利,鲟鱼的商业需求正在迅速增加,种间杂交代表了水产养殖生产的相关部分。在这项研究中,我们测试了扩增片段长度多态性(AFLP)技术的鲟鱼杂交检测的适用性。对多位点AFLP图谱进行了聚类分析和分配检验。我们的研究结果表明,这种方法可以很容易地识别鲟鱼杂交种,鼓励其应用不仅在鲟鱼,但也在其他系统组。
The identification of interspecific hybrids represents an important issue for conservation biology and trade controls. In Italy, the commercial demand for sturgeon is rapidly increasing and interspecific hybrids represent a relevant part of aquacultural production. In this study we tested the suitability of the amplified fragment length polymorphism (AFLP) technique for sturgeon hybrid detection. Multilocus AFLP profiles were analysed by cluster analysis and assignment tests based on observed and simulated samples. Our results show that this approach can easily identify sturgeon hybrids, encouraging its application not only in sturgeon but also in other systematic groups.